Surpassing light-induced cell damage in vitro with novel cell culture media

John H. Stockley; Kimberley Evans; Moritz Matthey; Katrin Volbracht; Sylvia Agathou; Jana Mukanowa; Juan Burrone; Ragnhildur T. Káradóttir

doi:10.1038/s41598-017-00829-x

Surpassing light-induced cell damage in vitro with novel cell culture media

John H. Stockley^*, Kimberley Evans, Moritz Matthey, Katrin Volbracht, Sylvia Agathou, Jana Mukanowa, Juan Burrone, Ragnhildur T. Káradóttir

^*Corresponding author for this work

Developmental Neurobiology

Wellcome Trust-Medical Research Council, Cambridge Stem Cell Institute

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

265 Downloads (Pure)

Abstract

Light is extensively used to study cells in real time (live cell imaging), separate cells using fluorescence activated cell sorting (FACS) and control cellular functions with light sensitive proteins (Optogenetics). However, photo-sensitive molecules inside cells and in standard cell culture media generate toxic by-products that interfere with cellular functions and cell viability when exposed to light. Here we show that primary cells from the rat central nervous system respond differently to photo-toxicity, in that astrocytes and microglia undergo morphological changes, while in developing neurons and oligodendrocyte progenitor cells (OPCs) it induces cellular death. To prevent photo-toxicity and to allow for long-term photo-stimulation without causing cellular damage, we formulated new photo-inert media called MEMO and NEUMO, and an antioxidant rich and serum free supplement called SOS. These new media reduced the detrimental effects caused by light and allowed cells to endure up to twenty times more light exposure without adverse effects, thus bypassing the optical constraints previously limiting experiments.

Original language	English
Article number	849
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-00829-x
Publication status	Published - 12 Apr 2017

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Surpassing light-induced cell_STOCKLEY_Publishedonline12April2017_GOLD VoR (CC-BY)Final published version, 11.1 MBLicence: CC BY

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AU - Agathou, Sylvia

AU - Mukanowa, Jana

AU - Burrone, Juan

AU - Káradóttir, Ragnhildur T.

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Surpassing light-induced cell damage in vitro with novel cell culture media

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