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Surprising differences in the respiratory protein of insects: A spectroscopic study of haemoglobin from the European honeybee and the malaria mosquito

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Surprising differences in the respiratory protein of insects: A spectroscopic study of haemoglobin from the European honeybee and the malaria mosquito. / Nys, Kevin; Cuypers, Bert; Berghmans, Herald; Hammerschmid, Dietmar; Moens, Luc; Dewilde, Sylvia; Van Doorslaer, Sabine.

In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1868, No. 6, 140413, 06.2020.

Research output: Contribution to journalArticle

Harvard

Nys, K, Cuypers, B, Berghmans, H, Hammerschmid, D, Moens, L, Dewilde, S & Van Doorslaer, S 2020, 'Surprising differences in the respiratory protein of insects: A spectroscopic study of haemoglobin from the European honeybee and the malaria mosquito', Biochimica et Biophysica Acta - Proteins and Proteomics, vol. 1868, no. 6, 140413.

APA

Nys, K., Cuypers, B., Berghmans, H., Hammerschmid, D., Moens, L., Dewilde, S., & Van Doorslaer, S. (2020). Surprising differences in the respiratory protein of insects: A spectroscopic study of haemoglobin from the European honeybee and the malaria mosquito. Biochimica et Biophysica Acta - Proteins and Proteomics, 1868(6), [140413].

Vancouver

Nys K, Cuypers B, Berghmans H, Hammerschmid D, Moens L, Dewilde S et al. Surprising differences in the respiratory protein of insects: A spectroscopic study of haemoglobin from the European honeybee and the malaria mosquito. Biochimica et Biophysica Acta - Proteins and Proteomics. 2020 Jun;1868(6). 140413.

Author

Nys, Kevin ; Cuypers, Bert ; Berghmans, Herald ; Hammerschmid, Dietmar ; Moens, Luc ; Dewilde, Sylvia ; Van Doorslaer, Sabine. / Surprising differences in the respiratory protein of insects: A spectroscopic study of haemoglobin from the European honeybee and the malaria mosquito. In: Biochimica et Biophysica Acta - Proteins and Proteomics. 2020 ; Vol. 1868, No. 6.

Bibtex Download

@article{fe45374c186049469fe21fb7e07c3f64,
title = "Surprising differences in the respiratory protein of insects: A spectroscopic study of haemoglobin from the European honeybee and the malaria mosquito",
abstract = "Only recently it was discovered that haemoglobin (Hb) belongs to the standard gene repertoire of insects, although their tracheal system is used for respiration. A classical oxygen-carrying function of Hb is only obvious for hexapods living in hypoxic environments. In other insect species, including the common fruit fly Drosophila melanogaster, the physiological role of Hb is yet unclear. Here, we study recombinant haemoglobin from the European honeybee Apis mellifera (Ame) and the malaria mosquito Anopheles gambiae (Aga). Spectroscopic evidence shows that both proteins can be classified as hexacoordinate Hbs with a strong affinity for the distal histidine. AgaHb1 is proposed to play a role in oxygen transport or sensing based on its multimeric state, slow autoxidation, and small but significant amount of five-coordinated haem in the deoxy ferrous form. AmeHb appears to behave more like vertebrate neuroglobin with a complex function given its diversified distribution in the genome.",
author = "Kevin Nys and Bert Cuypers and Herald Berghmans and Dietmar Hammerschmid and Luc Moens and Sylvia Dewilde and {Van Doorslaer}, Sabine",
year = "2020",
month = "6",
language = "English",
volume = "1868",
journal = "BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS",
issn = "1570-9639",
publisher = "Elsevier",
number = "6",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Surprising differences in the respiratory protein of insects: A spectroscopic study of haemoglobin from the European honeybee and the malaria mosquito

AU - Nys, Kevin

AU - Cuypers, Bert

AU - Berghmans, Herald

AU - Hammerschmid, Dietmar

AU - Moens, Luc

AU - Dewilde, Sylvia

AU - Van Doorslaer, Sabine

PY - 2020/6

Y1 - 2020/6

N2 - Only recently it was discovered that haemoglobin (Hb) belongs to the standard gene repertoire of insects, although their tracheal system is used for respiration. A classical oxygen-carrying function of Hb is only obvious for hexapods living in hypoxic environments. In other insect species, including the common fruit fly Drosophila melanogaster, the physiological role of Hb is yet unclear. Here, we study recombinant haemoglobin from the European honeybee Apis mellifera (Ame) and the malaria mosquito Anopheles gambiae (Aga). Spectroscopic evidence shows that both proteins can be classified as hexacoordinate Hbs with a strong affinity for the distal histidine. AgaHb1 is proposed to play a role in oxygen transport or sensing based on its multimeric state, slow autoxidation, and small but significant amount of five-coordinated haem in the deoxy ferrous form. AmeHb appears to behave more like vertebrate neuroglobin with a complex function given its diversified distribution in the genome.

AB - Only recently it was discovered that haemoglobin (Hb) belongs to the standard gene repertoire of insects, although their tracheal system is used for respiration. A classical oxygen-carrying function of Hb is only obvious for hexapods living in hypoxic environments. In other insect species, including the common fruit fly Drosophila melanogaster, the physiological role of Hb is yet unclear. Here, we study recombinant haemoglobin from the European honeybee Apis mellifera (Ame) and the malaria mosquito Anopheles gambiae (Aga). Spectroscopic evidence shows that both proteins can be classified as hexacoordinate Hbs with a strong affinity for the distal histidine. AgaHb1 is proposed to play a role in oxygen transport or sensing based on its multimeric state, slow autoxidation, and small but significant amount of five-coordinated haem in the deoxy ferrous form. AmeHb appears to behave more like vertebrate neuroglobin with a complex function given its diversified distribution in the genome.

M3 - Article

VL - 1868

JO - BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

JF - BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

SN - 1570-9639

IS - 6

M1 - 140413

ER -

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