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Sympathetic activation increases NO release from eNOS but neither eNOS nor nNOS play an essential role in exercise hyperemia in the human forearm

Research output: Contribution to journalArticle

Original languageEnglish
Article numberN/A
Pages (from-to)H1225-H1230
Number of pages6
JournalAMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
Volume304
Issue number9
DOIs
Publication statusPublished - 1 May 2013

King's Authors

Abstract

Nitric oxide (NO) release from endothelial NO synthase (eNOS) and/or neuronal NO synthase (nNOS) could be modulated by sympathetic nerve activity and contribute to increased blood flow after exercise. We examined the effects of brachial-arterial infusion of the nNOS selective inhibitor S-methyl-L-thiocitrulline (SMTC) and the nonselective NOS inhibitor N-G-monomethyl-L-arginine (L-NMMA) on forearm arm blood flow at rest, during sympathetic activation by lower body negative pressure, and during lower body negative pressure immediately after handgrip exercise. Reduction in forearm blood flow by lower body negative pressure during infusion of SMTC was not significantly different from that during vehicle (-28.5 +/- 4.02 vs. -34.1 +/- 2.96%, respectively; P = 0.32; n = 8). However, L-NMMA augmented the reduction in forearm blood flow by lower body negative pressure (-44.2 +/- 3.53 vs. -23.4 +/- 5.71%; n = 8; P <0.01). When lower body negative pressure was continued after handgrip exercise, there was no significant effect of either L-NMMA or SMTC on forearm blood flow immediately after low-intensity exercise (P = 0.91 and P = 0.44 for L-NMMA vs. saline and SMTC vs. saline, respectively; each n = 10) or high-intensity exercise (P = 0.46 and P = 0.68 for L-NMMA vs. saline and SMTC vs. saline, respectively; each n = 10). These results suggest that sympathetic activation increases NO release from eNOS, attenuating vasoconstriction. Dysfunction of eNOS could augment vasoconstrictor and blood pressure responses to sympathetic activation. However, neither eNOS nor nNOS plays an essential role in postexercise hyperaemia, even in the presence of increased sympathetic activation.

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