Synthesis, Characterization, and Application of Core–Shell Co0.16Fe2.84O4@NaYF4(Yb, Er) and Fe3O4@NaYF4(Yb, Tm) Nanoparticle as Trimodal (MRI, PET/SPECT, and Optical) Imaging Agents

Xianjin Cui, Domokos Mathe, Noémi Kovács, Ildikó Horváth, Maite Jauregui-Osoro, Rafael Torres Martin de Rosales, Gregory E. D. Mullen, Wilson Wong, Yong Yan, Dirk Krüger, Andrei N. Khlobystov, Maria Gimenez-Lopez, Mariann Semjeni, Krisztián Szigeti, Dániel S Veres, Haizhou Lu, Ignacio Hernández, William P. Gillin, Andrea Protti, Katalin Kis PetikMark A. Green, Philip J. Blower

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Multimodal nanoparticulate materials are described, offering magnetic, radionuclide, and fluorescent imaging capabilities to exploit the complementary advantages of magnetic resonance imaging (MRI), positron emission tomography/single-photon emission commuted tomography (PET/SPECT), and optical imaging. They comprise Fe3O4@NaYF4 core/shell nanoparticles (NPs) with different cation dopants in the shell or core, including Co0.16Fe2.84O4@NaYF4(Yb, Er) and Fe3O4@NaYF4(Yb, Tm). These NPs are stabilized by bisphosphonate polyethylene glycol conjugates (BP-PEG), and then show a high transverse relaxivity (r2) up to 326 mM–1 s–1 at 3T, a high affinity to [18F]-fluoride or radiometal-bisphosphonate conjugates (e.g., 64Cu and 99mTc), and fluorescent emissions from 500 to 800 nm under excitation at 980 nm. The biodistribution of intravenously administered particles determined by PET/MR imaging suggests that negatively charged Co0.16Fe2.84O4@NaYF4(Yb, Er)-BP-PEG (10K) NPs cleared from the blood pool more slowly than positively charged NPs Fe3O4@NaYF4(Yb, Tm)-BP-PEG (2K). Preliminary results in sentinel lymph node imaging in mice indicate the advantages of multimodal imaging.
Original languageEnglish
Pages (from-to)319-328
Number of pages10
JournalBioconjugate Chemistry
Issue number2
Early online date14 Jul 2015
Publication statusPublished - 17 Feb 2016

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