Synthesis, structural characterization, and quadruplex DNA binding studies of platinumd(II)-terpyridine complexes

TY - JOUR

T1 - Synthesis, structural characterization, and quadruplex DNA binding studies of platinumd(II)-terpyridine complexes

AU - Suntharalingam, Kogularamanan

AU - White, Andrew J P

AU - Vilar, Ramon

PY - 2009

Y1 - 2009

N2 - Three new substituted terpyrldine ligands and their corresponding platinum(ll) complexes have been prepared and fully characterized (including the X-ray crystal structures of two of the complexes). These compounds have shown to stack via π-π Interactions both In the solid state and in solution. The Interactions of these compounds with quadruplex DNA (both HTeIo and c-myc sequences) have been studied by fluorescent Indicator displacement (FID) assays, surface plasmon resonance (SPR), and In one case by circular dichrolsm (CD). The complexes have shown to interact strongly with quadruplex DNA (with binding constants of ca. 106 and DC50 values between 2.24 and 0.26, as determined by SPR and FID, respectively). We have also Investigated the Interaction between the complexes and a sequence of duplex DNA to gain some insight into the selectivity of the compounds for quadruplex structures. FID and SPR studies have shown that the complexes have a modest selectivity (1 order of magnitude) for quadruplex, particularly c-myc, versus duplex DNA.

AB - Three new substituted terpyrldine ligands and their corresponding platinum(ll) complexes have been prepared and fully characterized (including the X-ray crystal structures of two of the complexes). These compounds have shown to stack via π-π Interactions both In the solid state and in solution. The Interactions of these compounds with quadruplex DNA (both HTeIo and c-myc sequences) have been studied by fluorescent Indicator displacement (FID) assays, surface plasmon resonance (SPR), and In one case by circular dichrolsm (CD). The complexes have shown to interact strongly with quadruplex DNA (with binding constants of ca. 106 and DC50 values between 2.24 and 0.26, as determined by SPR and FID, respectively). We have also Investigated the Interaction between the complexes and a sequence of duplex DNA to gain some insight into the selectivity of the compounds for quadruplex structures. FID and SPR studies have shown that the complexes have a modest selectivity (1 order of magnitude) for quadruplex, particularly c-myc, versus duplex DNA.

UR - http://www.scopus.com/inward/record.url?scp=70349778727&partnerID=8YFLogxK

U2 - 10.1021/ic901319n

DO - 10.1021/ic901319n

M3 - Article

C2 - 19728717

AN - SCOPUS:70349778727

SN - 0020-1669

VL - 48

SP - 9427

EP - 9435

JO - INORGANIC CHEMISTRY

JF - INORGANIC CHEMISTRY

IS - 19

ER -