Synthetic Physical Interactions with the Yeast Centrosome

TY - JOUR

T1 - Synthetic Physical Interactions with the Yeast Centrosome

AU - Howell, Rowan S.M.

AU - Csikász-Nagy, Attila

AU - Thorpe, Peter H.

PY - 2019/7/9

Y1 - 2019/7/9

N2 - The yeast centrosome or Spindle Pole Body (SPB) is an organelle situated in the nuclear membrane, where it nucleates spindle microtubules and acts as a signaling hub. Various studies have explored the effects of forcing individual proteins to interact with the yeast SPB, however no systematic study has been performed. We used synthetic physical interactions to detect proteins that inhibit growth when forced to associate with the SPB. We found the SPB to be especially sensitive to relocalization, necessitating a novel data analysis approach. This novel analysis of SPI screening data shows that regions of the cell are locally more sensitive to forced relocalization than previously thought. Furthermore, we found a set of associations that result in elevated SPB number and, in some cases, multi-polar spindles. Since hyper-proliferation of centrosomes is a hallmark of cancer cells, these associations point the way for the use of yeast models in the study of spindle formation and chromosome segregation in cancer.

AB - The yeast centrosome or Spindle Pole Body (SPB) is an organelle situated in the nuclear membrane, where it nucleates spindle microtubules and acts as a signaling hub. Various studies have explored the effects of forcing individual proteins to interact with the yeast SPB, however no systematic study has been performed. We used synthetic physical interactions to detect proteins that inhibit growth when forced to associate with the SPB. We found the SPB to be especially sensitive to relocalization, necessitating a novel data analysis approach. This novel analysis of SPI screening data shows that regions of the cell are locally more sensitive to forced relocalization than previously thought. Furthermore, we found a set of associations that result in elevated SPB number and, in some cases, multi-polar spindles. Since hyper-proliferation of centrosomes is a hallmark of cancer cells, these associations point the way for the use of yeast models in the study of spindle formation and chromosome segregation in cancer.

KW - Body empirical Bayes

KW - Centrosome localization

KW - Spindle Pole

UR - http://www.scopus.com/inward/record.url?scp=85069328659&partnerID=8YFLogxK

U2 - 10.1534/g3.119.400117

DO - 10.1534/g3.119.400117

M3 - Article

C2 - 31076383

AN - SCOPUS:85069328659

SN - 2160-1836

VL - 9

SP - 2183

EP - 2194

JO - G3 (Bethesda, Md.)

JF - G3 (Bethesda, Md.)

IS - 7

ER -