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γδT cells but not αβT cells contribute to sepsis-induced white matter injury and motor abnormalities in mice

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γδT cells but not αβT cells contribute to sepsis-induced white matter injury and motor abnormalities in mice. / Zhang, Xiaoli; Rocha-Ferreira, Eridan; Li, Tao; Vontell, Regina; Jabin, Darakhshan; Hua, Sha; Zhou, Kai; Nazmi, Arshed; Albertsson, Anna-Maj; Sobotka, Kristina; Ek, Joakim; Thornton, Claire; Hagberg, Henrik; Mallard, Carina; Leavenworth, Jianmei W; Zhu, Changlian; Wang, Xiaoyang.

In: Journal of neuroinflammation, Vol. 14, No. 1, 20.12.2017, p. 255.

Research output: Contribution to journalArticle

Harvard

Zhang, X, Rocha-Ferreira, E, Li, T, Vontell, R, Jabin, D, Hua, S, Zhou, K, Nazmi, A, Albertsson, A-M, Sobotka, K, Ek, J, Thornton, C, Hagberg, H, Mallard, C, Leavenworth, JW, Zhu, C & Wang, X 2017, 'γδT cells but not αβT cells contribute to sepsis-induced white matter injury and motor abnormalities in mice', Journal of neuroinflammation, vol. 14, no. 1, pp. 255. https://doi.org/10.1186/s12974-017-1029-9

APA

Zhang, X., Rocha-Ferreira, E., Li, T., Vontell, R., Jabin, D., Hua, S., ... Wang, X. (2017). γδT cells but not αβT cells contribute to sepsis-induced white matter injury and motor abnormalities in mice. Journal of neuroinflammation, 14(1), 255. https://doi.org/10.1186/s12974-017-1029-9

Vancouver

Zhang X, Rocha-Ferreira E, Li T, Vontell R, Jabin D, Hua S et al. γδT cells but not αβT cells contribute to sepsis-induced white matter injury and motor abnormalities in mice. Journal of neuroinflammation. 2017 Dec 20;14(1):255. https://doi.org/10.1186/s12974-017-1029-9

Author

Zhang, Xiaoli ; Rocha-Ferreira, Eridan ; Li, Tao ; Vontell, Regina ; Jabin, Darakhshan ; Hua, Sha ; Zhou, Kai ; Nazmi, Arshed ; Albertsson, Anna-Maj ; Sobotka, Kristina ; Ek, Joakim ; Thornton, Claire ; Hagberg, Henrik ; Mallard, Carina ; Leavenworth, Jianmei W ; Zhu, Changlian ; Wang, Xiaoyang. / γδT cells but not αβT cells contribute to sepsis-induced white matter injury and motor abnormalities in mice. In: Journal of neuroinflammation. 2017 ; Vol. 14, No. 1. pp. 255.

Bibtex Download

@article{0cdcd035739940adb89dfb9aec40539d,
title = "γδT cells but not αβT cells contribute to sepsis-induced white matter injury and motor abnormalities in mice",
abstract = "BACKGROUND: Infection and sepsis are associated with brain white matter injury in preterm infants and the subsequent development of cerebral palsy.METHODS: In the present study, we used a neonatal mouse sepsis-induced white matter injury model to determine the contribution of different T cell subsets (αβT cells and γδT cells) to white matter injury and consequent behavioral changes. C57BL/6J wild-type (WT), T cell receptor (TCR) δ-deficient (Tcrd -/-, lacking γδT cells), and TCRα-deficient (Tcra -/-, lacking αβT cells) mice were administered with lipopolysaccharide (LPS) at postnatal day (PND) 2. Brain myelination was examined at PNDs 12, 26, and 60. Motor function and anxiety-like behavior were evaluated at PND 26 or 30 using DigiGait analysis and an elevated plus maze.RESULTS: White matter development was normal in Tcrd -/- and Tcrα -/- compared to WT mice. LPS exposure induced reductions in white matter tissue volume in WT and Tcrα -/- mice, but not in the Tcrd -/- mice, compared with the saline-treated groups. Neither LPS administration nor the T cell deficiency affected anxiety behavior in these mice as determined with the elevated plus maze. DigiGait analysis revealed motor function deficiency after LPS-induced sepsis in both WT and Tcrα -/- mice, but no such effect was observed in Tcrd -/- mice.CONCLUSIONS: Our results suggest that γδT cells but not αβT cells contribute to sepsis-induced white matter injury and subsequent motor function abnormalities in early life. Modulating the activity of γδT cells in the early stages of preterm white matter injury might represent a novel therapeutic strategy for the treatment of perinatal brain injury.",
author = "Xiaoli Zhang and Eridan Rocha-Ferreira and Tao Li and Regina Vontell and Darakhshan Jabin and Sha Hua and Kai Zhou and Arshed Nazmi and Anna-Maj Albertsson and Kristina Sobotka and Joakim Ek and Claire Thornton and Henrik Hagberg and Carina Mallard and Leavenworth, {Jianmei W} and Changlian Zhu and Xiaoyang Wang",
year = "2017",
month = "12",
day = "20",
doi = "10.1186/s12974-017-1029-9",
language = "English",
volume = "14",
pages = "255",
journal = "Journal of neuroinflammation",
issn = "1742-2094",
publisher = "BioMed Central",
number = "1",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - γδT cells but not αβT cells contribute to sepsis-induced white matter injury and motor abnormalities in mice

AU - Zhang, Xiaoli

AU - Rocha-Ferreira, Eridan

AU - Li, Tao

AU - Vontell, Regina

AU - Jabin, Darakhshan

AU - Hua, Sha

AU - Zhou, Kai

AU - Nazmi, Arshed

AU - Albertsson, Anna-Maj

AU - Sobotka, Kristina

AU - Ek, Joakim

AU - Thornton, Claire

AU - Hagberg, Henrik

AU - Mallard, Carina

AU - Leavenworth, Jianmei W

AU - Zhu, Changlian

AU - Wang, Xiaoyang

PY - 2017/12/20

Y1 - 2017/12/20

N2 - BACKGROUND: Infection and sepsis are associated with brain white matter injury in preterm infants and the subsequent development of cerebral palsy.METHODS: In the present study, we used a neonatal mouse sepsis-induced white matter injury model to determine the contribution of different T cell subsets (αβT cells and γδT cells) to white matter injury and consequent behavioral changes. C57BL/6J wild-type (WT), T cell receptor (TCR) δ-deficient (Tcrd -/-, lacking γδT cells), and TCRα-deficient (Tcra -/-, lacking αβT cells) mice were administered with lipopolysaccharide (LPS) at postnatal day (PND) 2. Brain myelination was examined at PNDs 12, 26, and 60. Motor function and anxiety-like behavior were evaluated at PND 26 or 30 using DigiGait analysis and an elevated plus maze.RESULTS: White matter development was normal in Tcrd -/- and Tcrα -/- compared to WT mice. LPS exposure induced reductions in white matter tissue volume in WT and Tcrα -/- mice, but not in the Tcrd -/- mice, compared with the saline-treated groups. Neither LPS administration nor the T cell deficiency affected anxiety behavior in these mice as determined with the elevated plus maze. DigiGait analysis revealed motor function deficiency after LPS-induced sepsis in both WT and Tcrα -/- mice, but no such effect was observed in Tcrd -/- mice.CONCLUSIONS: Our results suggest that γδT cells but not αβT cells contribute to sepsis-induced white matter injury and subsequent motor function abnormalities in early life. Modulating the activity of γδT cells in the early stages of preterm white matter injury might represent a novel therapeutic strategy for the treatment of perinatal brain injury.

AB - BACKGROUND: Infection and sepsis are associated with brain white matter injury in preterm infants and the subsequent development of cerebral palsy.METHODS: In the present study, we used a neonatal mouse sepsis-induced white matter injury model to determine the contribution of different T cell subsets (αβT cells and γδT cells) to white matter injury and consequent behavioral changes. C57BL/6J wild-type (WT), T cell receptor (TCR) δ-deficient (Tcrd -/-, lacking γδT cells), and TCRα-deficient (Tcra -/-, lacking αβT cells) mice were administered with lipopolysaccharide (LPS) at postnatal day (PND) 2. Brain myelination was examined at PNDs 12, 26, and 60. Motor function and anxiety-like behavior were evaluated at PND 26 or 30 using DigiGait analysis and an elevated plus maze.RESULTS: White matter development was normal in Tcrd -/- and Tcrα -/- compared to WT mice. LPS exposure induced reductions in white matter tissue volume in WT and Tcrα -/- mice, but not in the Tcrd -/- mice, compared with the saline-treated groups. Neither LPS administration nor the T cell deficiency affected anxiety behavior in these mice as determined with the elevated plus maze. DigiGait analysis revealed motor function deficiency after LPS-induced sepsis in both WT and Tcrα -/- mice, but no such effect was observed in Tcrd -/- mice.CONCLUSIONS: Our results suggest that γδT cells but not αβT cells contribute to sepsis-induced white matter injury and subsequent motor function abnormalities in early life. Modulating the activity of γδT cells in the early stages of preterm white matter injury might represent a novel therapeutic strategy for the treatment of perinatal brain injury.

U2 - 10.1186/s12974-017-1029-9

DO - 10.1186/s12974-017-1029-9

M3 - Article

C2 - 29262837

VL - 14

SP - 255

JO - Journal of neuroinflammation

JF - Journal of neuroinflammation

SN - 1742-2094

IS - 1

ER -

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