TY - JOUR
T1 - Tailoring the Architecture of Cationic Polymer Brush-Modified Carbon Nanotubes for Efficient siRNA Delivery in Cancer Immunotherapy
AU - Li, Danyang
AU - Ahmed, Momina
AU - Khan, Anisah
AU - Xu, Lizhou
AU - Walters, Adam A.
AU - Ballesteros, Belén
AU - Al-Jamal, Khuloud T.
N1 - Funding Information:
D.L. is a Maplethorpe Fellow. This project has received funding from the Maplethorpe Foundation, University of London, the Brain Tumour Charity (GN-000398), and Institutional Link-British Council (IL4337313). We thank Dr. Lim Yau for providing technical support of gel electrophoresis. ICN2 acknowledges the financial support from the Spanish Ministry of Economy and Competitiveness, through the “Severo Ochoa” Programme for Centres of Excellence in R&D (SEV-2017-0706). B.B. acknowledges funding from Generalitat de Catalunya 2017 SGR 327.
Publisher Copyright:
© 2021 American Chemical Society. All rights reserved.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/7/7
Y1 - 2021/7/7
N2 - The facile and controlled fabrication of homogeneously grafted cationic polymers on carbon nanotubes (CNTs) remains poorly investigated, which further hinders the understanding of interactions between functionalized CNTs with different nucleic acids and the rational design of appropriate gene delivery vehicles. Herein, we describe the controlled grafting of cationic poly(2-dimethylaminoethylmethacrylate) brushes on CNTs via surface-initiated atom transfer radical polymerization integrated with mussel-inspired polydopamine chemistry. The binding of nucleic acids with different brush-CNT hybrids discloses the highly architectural-dependent behavior with dense short brush-coated CNTs displaying the highest binding among all the other hybrids, namely, dense long, sparse long, and sparse short brush-coated CNTs. Additionally, different chemistries of the brush coatings were shown to influence the biocompatibility, cellular uptake, and silencing efficiency in vitro. This platform provides great flexibility for the design of polymer brush-CNT hybrids with precise control over their structure-activity relationship for the rational design of nucleic acid delivery systems.
AB - The facile and controlled fabrication of homogeneously grafted cationic polymers on carbon nanotubes (CNTs) remains poorly investigated, which further hinders the understanding of interactions between functionalized CNTs with different nucleic acids and the rational design of appropriate gene delivery vehicles. Herein, we describe the controlled grafting of cationic poly(2-dimethylaminoethylmethacrylate) brushes on CNTs via surface-initiated atom transfer radical polymerization integrated with mussel-inspired polydopamine chemistry. The binding of nucleic acids with different brush-CNT hybrids discloses the highly architectural-dependent behavior with dense short brush-coated CNTs displaying the highest binding among all the other hybrids, namely, dense long, sparse long, and sparse short brush-coated CNTs. Additionally, different chemistries of the brush coatings were shown to influence the biocompatibility, cellular uptake, and silencing efficiency in vitro. This platform provides great flexibility for the design of polymer brush-CNT hybrids with precise control over their structure-activity relationship for the rational design of nucleic acid delivery systems.
KW - atom transfer radical polymerization
KW - carbon nanotubes
KW - cationic polymer brush
KW - nucleic acid interaction
KW - polydopamine chemistry
KW - siRNA delivery
UR - http://www.scopus.com/inward/record.url?scp=85110309957&partnerID=8YFLogxK
U2 - 10.1021/acsami.1c02627
DO - 10.1021/acsami.1c02627
M3 - Article
AN - SCOPUS:85110309957
SN - 1944-8244
VL - 13
SP - 30284
EP - 30294
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 26
ER -
