Target Cell-Mediated Editing of HIV-1 cDNA by APOBEC3 Proteins in Human Macrophages

TY - JOUR

T1 - Target Cell-Mediated Editing of HIV-1 cDNA by APOBEC3 Proteins in Human Macrophages

AU - Koning, Fransje A.

AU - Goujon, Caroline

AU - Bauby, Helene

AU - Malim, Michael H.

PY - 2011/12

Y1 - 2011/12

N2 - Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3) proteins are encapsidated by assembling HIV-1 virions and edit viral cDNA in the next round of infection. Using alpha interferon (IFN-alpha)-treated monocyte-derived macrophages, we show that infrequent editing of HIV-1 reverse transcripts can also be mediated by APOBEC3 proteins supplied by the targets of infection. Based on the local sequence contexts of these mutations and the established characteristics of APOBEC3 protein expression in myeloid cells, we speculate that APOBEC3A may be responsible for a substantial proportion of this activity.

AB - Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3) proteins are encapsidated by assembling HIV-1 virions and edit viral cDNA in the next round of infection. Using alpha interferon (IFN-alpha)-treated monocyte-derived macrophages, we show that infrequent editing of HIV-1 reverse transcripts can also be mediated by APOBEC3 proteins supplied by the targets of infection. Based on the local sequence contexts of these mutations and the established characteristics of APOBEC3 protein expression in myeloid cells, we speculate that APOBEC3A may be responsible for a substantial proportion of this activity.

U2 - 10.1128/JVI.00775-11

DO - 10.1128/JVI.00775-11

M3 - Article

SN - 0022-538X

VL - 85

SP - 13448

EP - 13452

JO - Journal of Virology

JF - Journal of Virology

IS - 24

ER -