Telocytes regulate macrophages in periodontal disease

TY - JOUR

T1 - Telocytes regulate macrophages in periodontal disease

AU - Zhao, Jing

AU - Birjandi, Anahid A

AU - Ahmed, Mohi

AU - Redhead, Yushi

AU - Olea, Jose Villagomez

AU - Sharpe, Paul T

PY - 2022/10/4

Y1 - 2022/10/4

N2 - Telocytes (TCs) or interstitial cells are characterised in vivo by their long projections that contact other cell types. Although telocytes can be found in many different tissues including the heart , lung and intestine , their tissue-specific roles are poorly understood. Here we identify a specific cell signalling role for telocytes in the periodontium whereby telocytes regulate macrophage activity. We performed scRNA-seq and lineage tracing to identify telocytes and macrophages in mouse periodontium in homeostasis and periodontitis and carried out HGF signalling inhibition experiments using Tivantinib. We show that telocytes are quiescent in homeostasis, however, they proliferate and serve as a major source of HGF in periodontitis. Macrophages receive telocyte-derived HGF signals and shift from an M1 to a M1/M2 state. Our results reveal the source of HGF signals in periodontal tissue and provide new insights into the function of telocytes in regulating macrophage behaviour in periodontitis through HGF/Met cell signalling, that may provide a novel approach in periodontitis treatment. [Abstract copyright: © 2022, Zhao et al.]

AB - Telocytes (TCs) or interstitial cells are characterised in vivo by their long projections that contact other cell types. Although telocytes can be found in many different tissues including the heart , lung and intestine , their tissue-specific roles are poorly understood. Here we identify a specific cell signalling role for telocytes in the periodontium whereby telocytes regulate macrophage activity. We performed scRNA-seq and lineage tracing to identify telocytes and macrophages in mouse periodontium in homeostasis and periodontitis and carried out HGF signalling inhibition experiments using Tivantinib. We show that telocytes are quiescent in homeostasis, however, they proliferate and serve as a major source of HGF in periodontitis. Macrophages receive telocyte-derived HGF signals and shift from an M1 to a M1/M2 state. Our results reveal the source of HGF signals in periodontal tissue and provide new insights into the function of telocytes in regulating macrophage behaviour in periodontitis through HGF/Met cell signalling, that may provide a novel approach in periodontitis treatment. [Abstract copyright: © 2022, Zhao et al.]

KW - mouse

KW - cell biology

U2 - 10.7554/eLife.72128

DO - 10.7554/eLife.72128

M3 - Article

C2 - 36193890

SN - 2050-084X

VL - 11

JO - eLife

JF - eLife

ER -