The application of a Fasting-mimicking Diet in Periodontitis. A feasibility study

Giuseppe Mainas; Ipek Ozgu; Aysegul Sari; Manillo Vinciguerra; Mark Ide; Buse Bayraktar Ayakta; Kemal Ustun; Luigi Nibali

doi:10.1016/j.jdent.2025.105644

The application of a Fasting-mimicking Diet in Periodontitis. A feasibility study

Giuseppe Mainas, Ipek Ozgu, Aysegul Sari, Manillo Vinciguerra, Mark Ide, Buse Bayraktar Ayakta, Kemal Ustun, Luigi Nibali^*

^*Corresponding author for this work

Centre for Host Microbiome Interactions

Research output: Contribution to journal › Article › peer-review

Abstract

Objectives: The aim of this study was to test the feasibility of a fasting-mimicking diet on the systemic and periodontal response following non-surgical periodontal therapy. Methods: Twenty patients with periodontitis were randomised to receive steps 1 and 2 of periodontal treatment alone (following their normal diet) or with an adjunctive 5-day course of fasting-mimicking diet (FMD). Blood and gingival crevicular fluid (GCF) samples were collected to study the levels of several inflammatory biomarkers, along with clinical parameters and patients-reported outcome measurements (PROMs). All patients were followed up at day-1, day-7 and 3 months post-treatment, and food diaries were completed to assess their compliance. Results: Nineteen patients completed the 3-months follow-up. Only minimal adverse events including nausea, fatigue, weakness and dizziness were reported in the test group, with no differences in PROMs between groups. Test patients exhibited a non-statistically significant 3-months serum hs-CRP reduction of 0.20 ± 0.30 mg/l compared with 0.11 ± 0.52 mg/l in controls (p = 0.632) and a trend for lower GCF levels of MMP-8, IL-6 and IL-1β post-treatment compared with controls. Conclusions: This study suggests that one cycle of adjunctive FMD is feasible and may modulate the inflammatory response post-non-surgical periodontal therapy. Larger studies are needed to test this hypothesis. (clinicaltrials.gov ID: NCT05684627). Clinical relevance: For the first time, a 5-days cycle of FMD as an adjunct to non-surgical periodontal therapy was assessed in Turkish individuals with periodontitis stages III-IV. The findings showed that FMD is feasible and may reduce inflammatory markers one day post-treatment.

Original language	English
Article number	105644
Number of pages	20
Journal	Journal of Dentistry
Volume	156
Early online date	19 Feb 2025
DOIs	https://doi.org/10.1016/j.jdent.2025.105644
Publication status	E-pub ahead of print - 19 Feb 2025

Keywords

Fasting
periodontitis
Diet
Gingival Crevicular Fluid
serum

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title = "The application of a Fasting-mimicking Diet in Periodontitis. A feasibility study",

abstract = "Objectives: The aim of this study was to test the feasibility of a fasting-mimicking diet on the systemic and periodontal response following non-surgical periodontal therapy. Methods: Twenty patients with periodontitis were randomised to receive steps 1 and 2 of periodontal treatment alone (following their normal diet) or with an adjunctive 5-day course of fasting-mimicking diet (FMD). Blood and gingival crevicular fluid (GCF) samples were collected to study the levels of several inflammatory biomarkers, along with clinical parameters and patients-reported outcome measurements (PROMs). All patients were followed up at day-1, day-7 and 3 months post-treatment, and food diaries were completed to assess their compliance. Results: Nineteen patients completed the 3-months follow-up. Only minimal adverse events including nausea, fatigue, weakness and dizziness were reported in the test group, with no differences in PROMs between groups. Test patients exhibited a non-statistically significant 3-months serum hs-CRP reduction of 0.20 ± 0.30 mg/l compared with 0.11 ± 0.52 mg/l in controls (p = 0.632) and a trend for lower GCF levels of MMP-8, IL-6 and IL-1β post-treatment compared with controls. Conclusions: This study suggests that one cycle of adjunctive FMD is feasible and may modulate the inflammatory response post-non-surgical periodontal therapy. Larger studies are needed to test this hypothesis. (clinicaltrials.gov ID: NCT05684627). Clinical relevance: For the first time, a 5-days cycle of FMD as an adjunct to non-surgical periodontal therapy was assessed in Turkish individuals with periodontitis stages III-IV. The findings showed that FMD is feasible and may reduce inflammatory markers one day post-treatment.",

keywords = "Fasting, periodontitis, Diet, Gingival Crevicular Fluid, serum",

author = "Giuseppe Mainas and Ipek Ozgu and Aysegul Sari and Manillo Vinciguerra and Mark Ide and {Bayraktar Ayakta}, Buse and Kemal Ustun and Luigi Nibali",

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T1 - The application of a Fasting-mimicking Diet in Periodontitis. A feasibility study

AU - Mainas, Giuseppe

AU - Ozgu, Ipek

AU - Sari, Aysegul

AU - Vinciguerra, Manillo

AU - Ide, Mark

AU - Bayraktar Ayakta, Buse

AU - Ustun, Kemal

AU - Nibali, Luigi

PY - 2025/2/19

Y1 - 2025/2/19

N2 - Objectives: The aim of this study was to test the feasibility of a fasting-mimicking diet on the systemic and periodontal response following non-surgical periodontal therapy. Methods: Twenty patients with periodontitis were randomised to receive steps 1 and 2 of periodontal treatment alone (following their normal diet) or with an adjunctive 5-day course of fasting-mimicking diet (FMD). Blood and gingival crevicular fluid (GCF) samples were collected to study the levels of several inflammatory biomarkers, along with clinical parameters and patients-reported outcome measurements (PROMs). All patients were followed up at day-1, day-7 and 3 months post-treatment, and food diaries were completed to assess their compliance. Results: Nineteen patients completed the 3-months follow-up. Only minimal adverse events including nausea, fatigue, weakness and dizziness were reported in the test group, with no differences in PROMs between groups. Test patients exhibited a non-statistically significant 3-months serum hs-CRP reduction of 0.20 ± 0.30 mg/l compared with 0.11 ± 0.52 mg/l in controls (p = 0.632) and a trend for lower GCF levels of MMP-8, IL-6 and IL-1β post-treatment compared with controls. Conclusions: This study suggests that one cycle of adjunctive FMD is feasible and may modulate the inflammatory response post-non-surgical periodontal therapy. Larger studies are needed to test this hypothesis. (clinicaltrials.gov ID: NCT05684627). Clinical relevance: For the first time, a 5-days cycle of FMD as an adjunct to non-surgical periodontal therapy was assessed in Turkish individuals with periodontitis stages III-IV. The findings showed that FMD is feasible and may reduce inflammatory markers one day post-treatment.

AB - Objectives: The aim of this study was to test the feasibility of a fasting-mimicking diet on the systemic and periodontal response following non-surgical periodontal therapy. Methods: Twenty patients with periodontitis were randomised to receive steps 1 and 2 of periodontal treatment alone (following their normal diet) or with an adjunctive 5-day course of fasting-mimicking diet (FMD). Blood and gingival crevicular fluid (GCF) samples were collected to study the levels of several inflammatory biomarkers, along with clinical parameters and patients-reported outcome measurements (PROMs). All patients were followed up at day-1, day-7 and 3 months post-treatment, and food diaries were completed to assess their compliance. Results: Nineteen patients completed the 3-months follow-up. Only minimal adverse events including nausea, fatigue, weakness and dizziness were reported in the test group, with no differences in PROMs between groups. Test patients exhibited a non-statistically significant 3-months serum hs-CRP reduction of 0.20 ± 0.30 mg/l compared with 0.11 ± 0.52 mg/l in controls (p = 0.632) and a trend for lower GCF levels of MMP-8, IL-6 and IL-1β post-treatment compared with controls. Conclusions: This study suggests that one cycle of adjunctive FMD is feasible and may modulate the inflammatory response post-non-surgical periodontal therapy. Larger studies are needed to test this hypothesis. (clinicaltrials.gov ID: NCT05684627). Clinical relevance: For the first time, a 5-days cycle of FMD as an adjunct to non-surgical periodontal therapy was assessed in Turkish individuals with periodontitis stages III-IV. The findings showed that FMD is feasible and may reduce inflammatory markers one day post-treatment.

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KW - Gingival Crevicular Fluid

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DO - 10.1016/j.jdent.2025.105644

M3 - Article

SN - 0300-5712

VL - 156

JO - Journal of Dentistry

JF - Journal of Dentistry

M1 - 105644

ER -

The application of a Fasting-mimicking Diet in Periodontitis. A feasibility study

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Keywords

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