King's College London

Research portal

The assembly of developing motor neurons depends on an interplay between spontaneous activity, type II cadherins and gap junctions

Research output: Contribution to journalArticlepeer-review

Karli Montague, Andrew S Lowe, Ana Uzquiano, Athene Knüfer, Marc Astick, Stephen R Price, Sarah Guthrie

Original languageEnglish
Pages (from-to)830-836
Number of pages7
JournalDevelopment
Volume144
Issue number5
DOIs
Accepted/In press10 Jan 2017
Published1 Mar 2017

King's Authors

Abstract

A core structural and functional motif of the vertebrate central nervous system is discrete clusters of neurons or 'nuclei'. Yet the developmental mechanisms underlying this fundamental mode of organisation are largely unknown. We have previously shown that the assembly of motor neurons into nuclei depends on cadherin-mediated adhesion. Here, we demonstrate that the emergence of mature topography among motor nuclei involves a novel interplay between spontaneous activity, cadherin expression and gap junction communication. We report that nuclei display spontaneous calcium transients, and that changes in the activity patterns coincide with the course of nucleogenesis. We also find that these activity patterns are disrupted by manipulating cadherin or gap junction expression. Furthermore, inhibition of activity disrupts nucleogenesis, suggesting that activity feeds back to maintain integrity among motor neurons within a nucleus. Our study suggests that a network of interactions between cadherins, gap junctions and spontaneous activity governs neuron assembly, presaging circuit formation.

View graph of relations

© 2020 King's College London | Strand | London WC2R 2LS | England | United Kingdom | Tel +44 (0)20 7836 5454