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The Aurora B specificity switch is required to protect from non-disjunction at the metaphase/anaphase transition

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The Aurora B specificity switch is required to protect from non-disjunction at the metaphase/anaphase transition. / Kelly, Joanna R.; Martini, Silvia; Brownlow, Nicola; Joshi, Dhira; Federico, Stefania; Jamshidi, Shirin; Kjaer, Svend; Lockwood, Nicola; Rahman, Khondaker Miraz; Fraternali, Franca; Parker, Peter J.; Soliman, Tanya N.

In: Nature Communications, Vol. 11, 1396, 2020.

Research output: Contribution to journalArticle

Harvard

Kelly, JR, Martini, S, Brownlow, N, Joshi, D, Federico, S, Jamshidi, S, Kjaer, S, Lockwood, N, Rahman, KM, Fraternali, F, Parker, PJ & Soliman, TN 2020, 'The Aurora B specificity switch is required to protect from non-disjunction at the metaphase/anaphase transition', Nature Communications, vol. 11, 1396. https://doi.org/10.1038/s41467-020-15163-6

APA

Kelly, J. R., Martini, S., Brownlow, N., Joshi, D., Federico, S., Jamshidi, S., ... Soliman, T. N. (2020). The Aurora B specificity switch is required to protect from non-disjunction at the metaphase/anaphase transition. Nature Communications, 11, [1396]. https://doi.org/10.1038/s41467-020-15163-6

Vancouver

Kelly JR, Martini S, Brownlow N, Joshi D, Federico S, Jamshidi S et al. The Aurora B specificity switch is required to protect from non-disjunction at the metaphase/anaphase transition. Nature Communications. 2020;11. 1396. https://doi.org/10.1038/s41467-020-15163-6

Author

Kelly, Joanna R. ; Martini, Silvia ; Brownlow, Nicola ; Joshi, Dhira ; Federico, Stefania ; Jamshidi, Shirin ; Kjaer, Svend ; Lockwood, Nicola ; Rahman, Khondaker Miraz ; Fraternali, Franca ; Parker, Peter J. ; Soliman, Tanya N. / The Aurora B specificity switch is required to protect from non-disjunction at the metaphase/anaphase transition. In: Nature Communications. 2020 ; Vol. 11.

Bibtex Download

@article{a7121cb1c5c946c0bd67c303eb8d6f2c,
title = "The Aurora B specificity switch is required to protect from non-disjunction at the metaphase/anaphase transition",
abstract = "The Aurora B abscission checkpoint delays cytokinesis until resolution of DNA trapped in the cleavage furrow. This process involves PKCε phosphorylation of Aurora B S227. Assessing if this PKCε-Aurora B module provides a more widely exploited genome-protective control for the cell cycle, we show Aurora B phosphorylation at S227 by PKCε also occurs during mitosis. Expression of Aurora B S227A phenocopies inhibition of PKCε in by-passing the delay and resolution at anaphase entry that is associated with non-disjunction and catenation of sister chromatids. Implementation of this anaphase delay is reflected in PKCε activation following cell cycle dependent cleavage by caspase 7; knock-down of caspase 7 phenocopies PKCε loss, in a manner rescued by ectopically expressing/generating a free PKCε catalytic domain. Molecular dynamics indicates that Aurora B S227 phosphorylation induces conformational changes and this manifests in a profound switch in specificity towards S29 TopoIIα phosphorylation, a response necessary for catenation resolution during mitosis.",
author = "Kelly, {Joanna R.} and Silvia Martini and Nicola Brownlow and Dhira Joshi and Stefania Federico and Shirin Jamshidi and Svend Kjaer and Nicola Lockwood and Rahman, {Khondaker Miraz} and Franca Fraternali and Parker, {Peter J.} and Soliman, {Tanya N.}",
year = "2020",
doi = "10.1038/s41467-020-15163-6",
language = "English",
volume = "11",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - The Aurora B specificity switch is required to protect from non-disjunction at the metaphase/anaphase transition

AU - Kelly, Joanna R.

AU - Martini, Silvia

AU - Brownlow, Nicola

AU - Joshi, Dhira

AU - Federico, Stefania

AU - Jamshidi, Shirin

AU - Kjaer, Svend

AU - Lockwood, Nicola

AU - Rahman, Khondaker Miraz

AU - Fraternali, Franca

AU - Parker, Peter J.

AU - Soliman, Tanya N.

PY - 2020

Y1 - 2020

N2 - The Aurora B abscission checkpoint delays cytokinesis until resolution of DNA trapped in the cleavage furrow. This process involves PKCε phosphorylation of Aurora B S227. Assessing if this PKCε-Aurora B module provides a more widely exploited genome-protective control for the cell cycle, we show Aurora B phosphorylation at S227 by PKCε also occurs during mitosis. Expression of Aurora B S227A phenocopies inhibition of PKCε in by-passing the delay and resolution at anaphase entry that is associated with non-disjunction and catenation of sister chromatids. Implementation of this anaphase delay is reflected in PKCε activation following cell cycle dependent cleavage by caspase 7; knock-down of caspase 7 phenocopies PKCε loss, in a manner rescued by ectopically expressing/generating a free PKCε catalytic domain. Molecular dynamics indicates that Aurora B S227 phosphorylation induces conformational changes and this manifests in a profound switch in specificity towards S29 TopoIIα phosphorylation, a response necessary for catenation resolution during mitosis.

AB - The Aurora B abscission checkpoint delays cytokinesis until resolution of DNA trapped in the cleavage furrow. This process involves PKCε phosphorylation of Aurora B S227. Assessing if this PKCε-Aurora B module provides a more widely exploited genome-protective control for the cell cycle, we show Aurora B phosphorylation at S227 by PKCε also occurs during mitosis. Expression of Aurora B S227A phenocopies inhibition of PKCε in by-passing the delay and resolution at anaphase entry that is associated with non-disjunction and catenation of sister chromatids. Implementation of this anaphase delay is reflected in PKCε activation following cell cycle dependent cleavage by caspase 7; knock-down of caspase 7 phenocopies PKCε loss, in a manner rescued by ectopically expressing/generating a free PKCε catalytic domain. Molecular dynamics indicates that Aurora B S227 phosphorylation induces conformational changes and this manifests in a profound switch in specificity towards S29 TopoIIα phosphorylation, a response necessary for catenation resolution during mitosis.

UR - http://www.scopus.com/inward/record.url?scp=85081751670&partnerID=8YFLogxK

U2 - 10.1038/s41467-020-15163-6

DO - 10.1038/s41467-020-15163-6

M3 - Article

VL - 11

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 1396

ER -

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