The basement membrane of hair follicle stem cells is a muscle cell niche

TY - JOUR

T1 - The basement membrane of hair follicle stem cells is a muscle cell niche

AU - Fujiwara, Hironobu

AU - Ferreira, Manuela

AU - Donati, Giacomo

AU - Marciano, Denise K

AU - Linton, James M

AU - Sato, Yuya

AU - Hartner, Andrea

AU - Sekiguchi, Kiyotoshi

AU - Reichardt, Louis F

AU - Watt, Fiona M

N1 - Copyright © 2011 Elsevier Inc. All rights reserved.

PY - 2011/2/18

Y1 - 2011/2/18

N2 - The hair follicle bulge in the epidermis associates with the arrector pili muscle (APM) that is responsible for piloerection ("goosebumps"). We show that stem cells in the bulge deposit nephronectin into the underlying basement membrane, thus regulating the adhesion of mesenchymal cells expressing the nephronectin receptor, α8β1 integrin, to the bulge. Nephronectin induces α8 integrin-positive mesenchymal cells to upregulate smooth muscle markers. In nephronectin knockout mice, fewer arrector pili muscles form in the skin, and they attach to the follicle above the bulge, where there is compensatory upregulation of the nephronectin family member EGFL6. Deletion of α8 integrin also abolishes selective APM anchorage to the bulge. Nephronectin is a Wnt target; epidermal β-catenin activation upregulates epidermal nephronectin and dermal α8 integrin expression. Thus, bulge stem cells, via nephronectin expression, create a smooth muscle cell niche and act as tendon cells for the APM. Our results reveal a functional role for basement membrane heterogeneity in tissue patterning. PAPERCLIP:

AB - The hair follicle bulge in the epidermis associates with the arrector pili muscle (APM) that is responsible for piloerection ("goosebumps"). We show that stem cells in the bulge deposit nephronectin into the underlying basement membrane, thus regulating the adhesion of mesenchymal cells expressing the nephronectin receptor, α8β1 integrin, to the bulge. Nephronectin induces α8 integrin-positive mesenchymal cells to upregulate smooth muscle markers. In nephronectin knockout mice, fewer arrector pili muscles form in the skin, and they attach to the follicle above the bulge, where there is compensatory upregulation of the nephronectin family member EGFL6. Deletion of α8 integrin also abolishes selective APM anchorage to the bulge. Nephronectin is a Wnt target; epidermal β-catenin activation upregulates epidermal nephronectin and dermal α8 integrin expression. Thus, bulge stem cells, via nephronectin expression, create a smooth muscle cell niche and act as tendon cells for the APM. Our results reveal a functional role for basement membrane heterogeneity in tissue patterning. PAPERCLIP:

KW - Myocytes, Smooth Muscle

KW - Integrin alpha Chains

KW - Epidermis

KW - Animals

KW - Hair Follicle

KW - beta Catenin

KW - Extracellular Matrix Proteins

KW - Wnt Proteins

KW - Mice

KW - Gene Expression Regulation

KW - Basement Membrane

KW - Stem Cells

U2 - 10.1016/j.cell.2011.01.014

DO - 10.1016/j.cell.2011.01.014

M3 - Article

C2 - 21335239

SN - 0092-8674

VL - 144

SP - 577

EP - 589

JO - Cell

JF - Cell

IS - 4

ER -