The calcium influx pathway in rat olfactory ensheathing cells shows TRPC channel pharmacology

R Davies; S Hayat; C B Wigley; J Robbins

doi:10.1016/j.brainres.2004.07.032

The calcium influx pathway in rat olfactory ensheathing cells shows TRPC channel pharmacology

R Davies, S Hayat, C B Wigley, J Robbins

Wolfson SPaRC

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Pharmacological characterisation of the calcium influx pathway in olfactory ensheathing bells (OECs) was performed using Indo-1 calcium microfluorometry. Our previous work has implicated this pathway in olfactory ensheathing cell support for regeneration of axons from adult CNS neurons. In high extracellular calcium (20 mM), cumulative concentration inhibition curves were generated for Lu3+, Gd3+ and econazole, giving IC(50)s of 0.09, 1.51 and 1.13 muM, respectively, and slope values that were not significantly different from unity. Combining these results with those obtained previously, an order of inhibitor potency was found to be Lu3+>La3+=econazole=Gd3+>1-[2-(4-methoxyphenyl)-2-[3-(4-methoxyphenyl)pr opoxy]ethyl-1H-imidazole hydrochloride (SKF96365)>Cd2+. This profile most closely fits some members of the TRPC family of non-voltage gated calcium influx channels and may indicate that a TRP-mediated calcium influx plays a role in glial-neuronal interaction and axonal regeneration. (C) 2004 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	154 - 156
Number of pages	3
Journal	Brain Research
Volume	1023
Issue number	1
DOIs	https://doi.org/10.1016/j.brainres.2004.07.032
Publication status	Published - 8 Oct 2004

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10.1016/j.brainres.2004.07.032

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title = "The calcium influx pathway in rat olfactory ensheathing cells shows TRPC channel pharmacology",

abstract = "Pharmacological characterisation of the calcium influx pathway in olfactory ensheathing bells (OECs) was performed using Indo-1 calcium microfluorometry. Our previous work has implicated this pathway in olfactory ensheathing cell support for regeneration of axons from adult CNS neurons. In high extracellular calcium (20 mM), cumulative concentration inhibition curves were generated for Lu3+, Gd3+ and econazole, giving IC(50)s of 0.09, 1.51 and 1.13 muM, respectively, and slope values that were not significantly different from unity. Combining these results with those obtained previously, an order of inhibitor potency was found to be Lu3+>La3+=econazole=Gd3+>1-[2-(4-methoxyphenyl)-2-[3-(4-methoxyphenyl)pr opoxy]ethyl-1H-imidazole hydrochloride (SKF96365)>Cd2+. This profile most closely fits some members of the TRPC family of non-voltage gated calcium influx channels and may indicate that a TRP-mediated calcium influx plays a role in glial-neuronal interaction and axonal regeneration. (C) 2004 Elsevier B.V. All rights reserved.",

author = "R Davies and S Hayat and Wigley, {C B} and J Robbins",

year = "2004",

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doi = "10.1016/j.brainres.2004.07.032",

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T1 - The calcium influx pathway in rat olfactory ensheathing cells shows TRPC channel pharmacology

AU - Davies, R

AU - Hayat, S

AU - Wigley, C B

AU - Robbins, J

PY - 2004/10/8

Y1 - 2004/10/8

N2 - Pharmacological characterisation of the calcium influx pathway in olfactory ensheathing bells (OECs) was performed using Indo-1 calcium microfluorometry. Our previous work has implicated this pathway in olfactory ensheathing cell support for regeneration of axons from adult CNS neurons. In high extracellular calcium (20 mM), cumulative concentration inhibition curves were generated for Lu3+, Gd3+ and econazole, giving IC(50)s of 0.09, 1.51 and 1.13 muM, respectively, and slope values that were not significantly different from unity. Combining these results with those obtained previously, an order of inhibitor potency was found to be Lu3+>La3+=econazole=Gd3+>1-[2-(4-methoxyphenyl)-2-[3-(4-methoxyphenyl)pr opoxy]ethyl-1H-imidazole hydrochloride (SKF96365)>Cd2+. This profile most closely fits some members of the TRPC family of non-voltage gated calcium influx channels and may indicate that a TRP-mediated calcium influx plays a role in glial-neuronal interaction and axonal regeneration. (C) 2004 Elsevier B.V. All rights reserved.

AB - Pharmacological characterisation of the calcium influx pathway in olfactory ensheathing bells (OECs) was performed using Indo-1 calcium microfluorometry. Our previous work has implicated this pathway in olfactory ensheathing cell support for regeneration of axons from adult CNS neurons. In high extracellular calcium (20 mM), cumulative concentration inhibition curves were generated for Lu3+, Gd3+ and econazole, giving IC(50)s of 0.09, 1.51 and 1.13 muM, respectively, and slope values that were not significantly different from unity. Combining these results with those obtained previously, an order of inhibitor potency was found to be Lu3+>La3+=econazole=Gd3+>1-[2-(4-methoxyphenyl)-2-[3-(4-methoxyphenyl)pr opoxy]ethyl-1H-imidazole hydrochloride (SKF96365)>Cd2+. This profile most closely fits some members of the TRPC family of non-voltage gated calcium influx channels and may indicate that a TRP-mediated calcium influx plays a role in glial-neuronal interaction and axonal regeneration. (C) 2004 Elsevier B.V. All rights reserved.

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DO - 10.1016/j.brainres.2004.07.032

M3 - Article

SN - 1873-2747

VL - 1023

SP - 154

EP - 156

JO - Brain Research

JF - Brain Research

IS - 1

ER -

The calcium influx pathway in rat olfactory ensheathing cells shows TRPC channel pharmacology

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