The citrus flavanone naringenin inhibits inflammatory signalling in glial cells and protects against neuroinflammatory injury

Katerina Vafeiadou, David Vauzour, Hung Yi Lee, Ana Rodriguez-Mateos, Robert J Williams, Jeremy P E Spencer

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205 Citations (Scopus)


Neuroinflammation plays an integral role in the progression of neurodegeneration. In this study we investigated the anti-inflammatory effects of different classes of flavonoids (flavanones, flavanols and anthocyanidins) in primary mixed glial cells. We found that the flavanones naringenin and hesperetin and the flavanols (+)-catechin and (-)-epicatechin, but not the anthocyanidins cyanidin and pelargonidin, attenuated LPS/IFN-gamma-induced TNF-alpha production in glial cells. Naringenin also inhibited LPS/IFN-gamma-induced iNOS expression and nitric oxide production in glial cells, thus showing the strongest anti-inflammatory activity among all flavonoids tested. Moreover, naringenin protected against inflammatory-induced neuronal death in a primary neuronal-glial co-culture system. Naringenin also inhibited LPS/IFN-gamma-induced p38 mitogen-activated protein kinase (MAPK) phosphorylation and downstream signal transducer and activator of transcription-1 (STAT-1) in LPS/IFN-gamma stimulated primary mixed glial cells. Taken together, our results suggest that naringenin may produce an anti-inflammatory effect in LPS/IFN-gamma stimulated glial cells that may be due to its interaction with p38 signalling cascades and the STAT-1 transcription factor.

Original languageEnglish
Pages (from-to)100-9
Number of pages10
JournalArchives of biochemistry and biophysics
Issue number1
Publication statusPublished - 1 Apr 2009


  • Animals
  • Cells, Cultured
  • Coculture Techniques
  • Flavanones
  • Hesperidin
  • Inflammation
  • Interferon-gamma
  • Lipopolysaccharides
  • Mice
  • Neuroglia
  • Neurons
  • Phosphorylation
  • STAT1 Transcription Factor
  • Signal Transduction
  • p38 Mitogen-Activated Protein Kinases

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