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“The devil's in the detail”: Release of an expanded, enhanced and dynamically revised forensic STR Sequence Guide

Research output: Contribution to journalArticlepeer-review

C. Phillips, K. Butler Gettings, Jonathan L King, D. Ballard, M. Bodner, L. Borsuk, Walther Parson

Original languageEnglish
Pages (from-to)162-169
Number of pages8
JournalForensic Science International: Genetics
Early online date21 Feb 2018
Accepted/In press18 Feb 2018
E-pub ahead of print21 Feb 2018
Published1 May 2018


King's Authors


The STR sequence template file published in 2016 as part of the considerations from the DNA Commission of the International Society for Forensic Genetics on minimal STR sequence nomenclature requirements, has been comprehensively revised and audited using the latest GRCh38 genome assembly. The list of forensic STRs characterized was expanded by including supplementary autosomal, X- and Y-chromosome microsatellites in less common use for routine DNA profiling, but some likely to be adopted in future massively parallel sequencing (MPS) STR panels. We outline several aspects of sequence alignment and annotation that required care and attention to detail when comparing sequences to GRCh37 and GRCh38 assemblies, as well as the necessary matching of MPS-based allele descriptions to previously established repeat region structures described in initial sequencing studies of the less well known forensic STRs. The revised sequence guide is now available in a dynamically updated FTP format from the STRidER website with a date-stamped change log to allow users to explore their own MPS data with the most up-to-date forensic STR sequence information compiled in a simple guide.

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