The distribution and quantitation of cementum-bound lipopolysaccharide on periodontally diseased root surfaces of human teeth

TY - JOUR

T1 - The distribution and quantitation of cementum-bound lipopolysaccharide on periodontally diseased root surfaces of human teeth

AU - Hughes, F J

AU - Smales, F C

AU - Hughes, Francis

PY - 1990

Y1 - 1990

N2 - The aim of this study was to estimate the concentration of cementum-bound LPS on a group of 12 teeth that had been extracted because of periodontitis. LPS on scaled root surfaces was labelled by immunogold/silver staining. The concentrations of LPS were estimated by quantifying the amount of bound silver label, using X-ray microanalysis in areas free of plaque or calculus. These were compared against standards of known LPS concentration, which were separately prepared for each sample. Cementum-bound LPS was detected at concentrations of up to 2 EU/mm2 of affected root surface. However, most of the root surfaces had considerably lower concentrations than this, the mean of all samples never exceeding 0.7 EU/mm2. LPS concentrations were highest on cementum towards the apical regions of the affected pocket. These findings confirm that cementum-bound LPS is only present in low concentrations on affected teeth, and suggest that the clinical significance of cementum-associated LPS may have been over-estimated in the past. The demonstration of LPS appears to be more important as an indicator of retained bacteria and calculus than of cementum-bound LPS per se.

AB - The aim of this study was to estimate the concentration of cementum-bound LPS on a group of 12 teeth that had been extracted because of periodontitis. LPS on scaled root surfaces was labelled by immunogold/silver staining. The concentrations of LPS were estimated by quantifying the amount of bound silver label, using X-ray microanalysis in areas free of plaque or calculus. These were compared against standards of known LPS concentration, which were separately prepared for each sample. Cementum-bound LPS was detected at concentrations of up to 2 EU/mm2 of affected root surface. However, most of the root surfaces had considerably lower concentrations than this, the mean of all samples never exceeding 0.7 EU/mm2. LPS concentrations were highest on cementum towards the apical regions of the affected pocket. These findings confirm that cementum-bound LPS is only present in low concentrations on affected teeth, and suggest that the clinical significance of cementum-associated LPS may have been over-estimated in the past. The demonstration of LPS appears to be more important as an indicator of retained bacteria and calculus than of cementum-bound LPS per se.

KW - Dental Cementum

KW - Electron Probe Microanalysis

KW - Humans

KW - Immunohistochemistry

KW - Lipopolysaccharides

KW - Periodontitis

KW - Tooth Root

M3 - Article

C2 - 2378584

SN - 0003-9969

VL - 35

SP - 295

EP - 299

JO - Archives of Oral Biology

JF - Archives of Oral Biology

IS - 4

ER -