TY - JOUR
T1 - The effect of atherosclerosis on neuromodulation of sympathetic neurotransmission by neuropeptide Y and calcitonin gene-related peptide in the rabbit mesenteric artery
AU - Stewart-Lee, Anne L.
AU - Aberdeen, Judy
AU - Burnstock, Geoffrey
PY - 1992/6/5
Y1 - 1992/6/5
N2 - The neuromodulatory actions of neuropeptide Y (NPY) (0.1 μM) and calciton gene-related peptide (CGRP) (0.01 μM) on nerve-evoked contractions have been studied in the Watanabe heritable hyperlipidemic (WHHL) rabbit mesenteric artery from 4-, 6- and 12-month-old animals with New Zealand white (NZW) rabbits being used as age- and sex-matched controls. Nerve-evoked contractions in 12-month-old rabbits were smaller in WHHL in comparison to NZW rabbits, with no difference between the two strains of rabbit at 4 and 6 months of age. Both the potentiating effect of NPY and the inhibitory effect of CGRP on nerve-evoked contractions increased significantly at 12 months of age compared with responses measured in younger WHHL rabbits, and were greater than in 12-month-old control NZW rabbits. In contrast, the direct smooth muscle relaxant response of CGRP on raised-tone preparations was not different between the two strains of rabbit at any age. Both NPY-immunoreactive and CGRP-immunoreactive nerve fibres were less varicose in 6- and 12-month-old WHHL rabbits when compared with younger WHHL rabbits and NZW controls. In conclusion, this study shows that while nerve-evoked contractions are reduced, in the 12-month-old WHHL rabbit mesenteric artery, the neuromodulatory actions of NPY and CGRP are augmented.
AB - The neuromodulatory actions of neuropeptide Y (NPY) (0.1 μM) and calciton gene-related peptide (CGRP) (0.01 μM) on nerve-evoked contractions have been studied in the Watanabe heritable hyperlipidemic (WHHL) rabbit mesenteric artery from 4-, 6- and 12-month-old animals with New Zealand white (NZW) rabbits being used as age- and sex-matched controls. Nerve-evoked contractions in 12-month-old rabbits were smaller in WHHL in comparison to NZW rabbits, with no difference between the two strains of rabbit at 4 and 6 months of age. Both the potentiating effect of NPY and the inhibitory effect of CGRP on nerve-evoked contractions increased significantly at 12 months of age compared with responses measured in younger WHHL rabbits, and were greater than in 12-month-old control NZW rabbits. In contrast, the direct smooth muscle relaxant response of CGRP on raised-tone preparations was not different between the two strains of rabbit at any age. Both NPY-immunoreactive and CGRP-immunoreactive nerve fibres were less varicose in 6- and 12-month-old WHHL rabbits when compared with younger WHHL rabbits and NZW controls. In conclusion, this study shows that while nerve-evoked contractions are reduced, in the 12-month-old WHHL rabbit mesenteric artery, the neuromodulatory actions of NPY and CGRP are augmented.
KW - Atherosclerosis
KW - CGRP (calcitonin gene-related peptide)
KW - Mesenteric artery
KW - Neuropeptide Y (NPY)
KW - Sympathetic neurotransmission
KW - WHHL rabbit
U2 - 10.1016/0014-2999(92)90357-A
DO - 10.1016/0014-2999(92)90357-A
M3 - Article
C2 - 1327811
AN - SCOPUS:0026634680
SN - 0014-2999
VL - 216
SP - 167
EP - 174
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 2
ER -