The effect of experimental conditions on the detection of spermine in cell extracts and tissues

TY - JOUR

T1 - The effect of experimental conditions on the detection of spermine in cell extracts and tissues

AU - Spencer, NG

AU - Eykyn, TR

AU - DeSouza, NM

AU - Payne, GS

PY - 2010/2

Y1 - 2010/2

N2 - The aim of this work was to investigate the effect of experimental conditions on the visibility of polyamines. In solution the chemical shift of the three groups of peaks (at approximately 1.8, 2.1 and 3.1 ppm) were found to be pH dependent. Relaxation times in aqueous solution at pH 7.0, 298K and 11.74T were measured to be: putrescine (T-1 = 2.495, T-2 = 2.07 s), spermidine (T-1 = 1.27 s, T-2 = 1.055) and spermine (T-1 = 1.02s, T-2 = 0.82s). Simple spin-echo sequences could not be used to measure T-2 as the spins also experience phase evolution from homonuclear coupling which imposes a modulation on the T-2 decay curve. This modulation is eliminated by using CPMG sequences with an echo spacing of

AB - The aim of this work was to investigate the effect of experimental conditions on the visibility of polyamines. In solution the chemical shift of the three groups of peaks (at approximately 1.8, 2.1 and 3.1 ppm) were found to be pH dependent. Relaxation times in aqueous solution at pH 7.0, 298K and 11.74T were measured to be: putrescine (T-1 = 2.495, T-2 = 2.07 s), spermidine (T-1 = 1.27 s, T-2 = 1.055) and spermine (T-1 = 1.02s, T-2 = 0.82s). Simple spin-echo sequences could not be used to measure T-2 as the spins also experience phase evolution from homonuclear coupling which imposes a modulation on the T-2 decay curve. This modulation is eliminated by using CPMG sequences with an echo spacing of

M3 - Article

VL - 23

SP - 163

EP - 169

JO - Nmr in Biomedicine

JF - Nmr in Biomedicine

IS - 2

ER -