The effect of monensin on cell aggregation of normal and dystrophic human skin fibroblasts

G. E. Jones; J. A. Pizzey; J. A. Witkowski

doi:10.1016/S0014-4827(85)80028-8

The effect of monensin on cell aggregation of normal and dystrophic human skin fibroblasts

G. E. Jones^*, J. A. Pizzey, J. A. Witkowski

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Measurements of aggregation kinetics using couette viscometry show that freshly trypsinized skin fibroblasts from patients with Duchenne muscular dystrophy have values of intercellular adhesiveness approx. 40% those of normal cells. If cells are allowed to recover from the effects of trypsinization (by incubation for 2 h at 37°C in serum-containing medium) the intercellular adhesiveness of both cell types increases, and normal and Duchenne cells aggregate to the same extent. Exposure to the ionophore monensin during the recovery phase leads to suppression of recovery in both cell types, and this effect of the drug is greater in Duchenne fibroblasts. These results are discussed in relation to other data on the reported differential effects of trypsin and monensin on normal and Duchenne fibroblasts.

Original language	English
Pages (from-to)	540-545
Number of pages	6
Journal	Experimental Cell Research
Volume	159
Issue number	2
DOIs	https://doi.org/10.1016/S0014-4827(85)80028-8
Publication status	Published - 1 Jan 1985

Access to Document

10.1016/S0014-4827(85)80028-8

Cite this

@article{4b82ea7148c24791bc915beb041f891f,

title = "The effect of monensin on cell aggregation of normal and dystrophic human skin fibroblasts",

abstract = "Measurements of aggregation kinetics using couette viscometry show that freshly trypsinized skin fibroblasts from patients with Duchenne muscular dystrophy have values of intercellular adhesiveness approx. 40% those of normal cells. If cells are allowed to recover from the effects of trypsinization (by incubation for 2 h at 37°C in serum-containing medium) the intercellular adhesiveness of both cell types increases, and normal and Duchenne cells aggregate to the same extent. Exposure to the ionophore monensin during the recovery phase leads to suppression of recovery in both cell types, and this effect of the drug is greater in Duchenne fibroblasts. These results are discussed in relation to other data on the reported differential effects of trypsin and monensin on normal and Duchenne fibroblasts.",

author = "Jones, {G. E.} and Pizzey, {J. A.} and Witkowski, {J. A.}",

year = "1985",

month = jan,

day = "1",

doi = "10.1016/S0014-4827(85)80028-8",

language = "English",

volume = "159",

pages = "540--545",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "ACADEMIC PRESS INC",

number = "2",

}

TY - JOUR

T1 - The effect of monensin on cell aggregation of normal and dystrophic human skin fibroblasts

AU - Jones, G. E.

AU - Pizzey, J. A.

AU - Witkowski, J. A.

PY - 1985/1/1

Y1 - 1985/1/1

N2 - Measurements of aggregation kinetics using couette viscometry show that freshly trypsinized skin fibroblasts from patients with Duchenne muscular dystrophy have values of intercellular adhesiveness approx. 40% those of normal cells. If cells are allowed to recover from the effects of trypsinization (by incubation for 2 h at 37°C in serum-containing medium) the intercellular adhesiveness of both cell types increases, and normal and Duchenne cells aggregate to the same extent. Exposure to the ionophore monensin during the recovery phase leads to suppression of recovery in both cell types, and this effect of the drug is greater in Duchenne fibroblasts. These results are discussed in relation to other data on the reported differential effects of trypsin and monensin on normal and Duchenne fibroblasts.

AB - Measurements of aggregation kinetics using couette viscometry show that freshly trypsinized skin fibroblasts from patients with Duchenne muscular dystrophy have values of intercellular adhesiveness approx. 40% those of normal cells. If cells are allowed to recover from the effects of trypsinization (by incubation for 2 h at 37°C in serum-containing medium) the intercellular adhesiveness of both cell types increases, and normal and Duchenne cells aggregate to the same extent. Exposure to the ionophore monensin during the recovery phase leads to suppression of recovery in both cell types, and this effect of the drug is greater in Duchenne fibroblasts. These results are discussed in relation to other data on the reported differential effects of trypsin and monensin on normal and Duchenne fibroblasts.

UR - http://www.scopus.com/inward/record.url?scp=0021965750&partnerID=8YFLogxK

U2 - 10.1016/S0014-4827(85)80028-8

DO - 10.1016/S0014-4827(85)80028-8

M3 - Article

C2 - 4029279

AN - SCOPUS:0021965750

SN - 0014-4827

VL - 159

SP - 540

EP - 545

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

The effect of monensin on cell aggregation of normal and dystrophic human skin fibroblasts

Abstract

Access to Document

Other files and links

Fingerprint

Cite this