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The effect of phloretin on synaptic proteins and adult hippocampal neurogenesis in Aβ (1-42)-injected male Wistar rats

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The effect of phloretin on synaptic proteins and adult hippocampal neurogenesis in Aβ (1-42)-injected male Wistar rats. / Ghumatkar, Priya; Peshattiwar, Vaibhavi; Patil, Sachin; Muke, Suraj; Whitfield, David; Howlett, David; Francis, Paul; Sathaye, Sadhana.

In: Journal of Pharmacy and Pharmacology, Vol. 70, No. 8, 08.2018, p. 1022-1030.

Research output: Contribution to journalArticle

Harvard

Ghumatkar, P, Peshattiwar, V, Patil, S, Muke, S, Whitfield, D, Howlett, D, Francis, P & Sathaye, S 2018, 'The effect of phloretin on synaptic proteins and adult hippocampal neurogenesis in Aβ (1-42)-injected male Wistar rats', Journal of Pharmacy and Pharmacology, vol. 70, no. 8, pp. 1022-1030. https://doi.org/10.1111/jphp.12925

APA

Ghumatkar, P., Peshattiwar, V., Patil, S., Muke, S., Whitfield, D., Howlett, D., ... Sathaye, S. (2018). The effect of phloretin on synaptic proteins and adult hippocampal neurogenesis in Aβ (1-42)-injected male Wistar rats. Journal of Pharmacy and Pharmacology, 70(8), 1022-1030. https://doi.org/10.1111/jphp.12925

Vancouver

Ghumatkar P, Peshattiwar V, Patil S, Muke S, Whitfield D, Howlett D et al. The effect of phloretin on synaptic proteins and adult hippocampal neurogenesis in Aβ (1-42)-injected male Wistar rats. Journal of Pharmacy and Pharmacology. 2018 Aug;70(8):1022-1030. https://doi.org/10.1111/jphp.12925

Author

Ghumatkar, Priya ; Peshattiwar, Vaibhavi ; Patil, Sachin ; Muke, Suraj ; Whitfield, David ; Howlett, David ; Francis, Paul ; Sathaye, Sadhana. / The effect of phloretin on synaptic proteins and adult hippocampal neurogenesis in Aβ (1-42)-injected male Wistar rats. In: Journal of Pharmacy and Pharmacology. 2018 ; Vol. 70, No. 8. pp. 1022-1030.

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@article{e58ddc38d4134d8d8a2d11e792f98c4d,
title = "The effect of phloretin on synaptic proteins and adult hippocampal neurogenesis in Aβ (1-42)-injected male Wistar rats",
abstract = "Objectives: Considering the deleterious effect of Aβ1-42, a study was designed to evaluate the effect of phloretin on altered synaptic proteins and adult hippocampal neurogenesis in Aβ1-42-injected Wistar rats. Methods: The rats were pretreated with 5 mg/kg p.o dose of phloretin and donepezil (positive control) for 28 days, followed by intrahippocampal injections of aggregated Aβ1-42. After termination, perfused brains were isolated and subjected to Western blot and immunohistochemistry (IHC) analysis. Key findings: The Western blot revealed that Aβ1-42-injected rats had significantly low levels of synaptophysin as compared to sham control. Phloretin pretreatment significantly protected the presynaptic protein synaptophysin against the effects of Aβ1-42. There were no significant changes in the levels of PSD95 between different groups. The IHC findings showed that Aβ1-42 significantly reduced the Ki67 and DCX in the dentate gyrus as compared to sham control. However, phloretin significantly improved the number of Ki67- and DCX-positive neurons in the dentate gyrus region as compared to Aβ1-42 group. Conclusions: This study demonstrated the protective effect of phloretin on synaptophysin and adult neuronal proliferating cells in Aβ1-42-injected rats. The encouraging findings highlight the potential of phloretin as a dietary supplement targeting key therapeutic mechanisms in neurodegenerative disorders such as AD.",
keywords = "antioxidants, flavonoids, neurogenesis, phloretin, synaptophysin",
author = "Priya Ghumatkar and Vaibhavi Peshattiwar and Sachin Patil and Suraj Muke and David Whitfield and David Howlett and Paul Francis and Sadhana Sathaye",
year = "2018",
month = "8",
doi = "10.1111/jphp.12925",
language = "English",
volume = "70",
pages = "1022--1030",
journal = "Journal of Pharmacy and Pharmacology",
issn = "0022-3573",
publisher = "Wiley-Blackwell",
number = "8",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - The effect of phloretin on synaptic proteins and adult hippocampal neurogenesis in Aβ (1-42)-injected male Wistar rats

AU - Ghumatkar, Priya

AU - Peshattiwar, Vaibhavi

AU - Patil, Sachin

AU - Muke, Suraj

AU - Whitfield, David

AU - Howlett, David

AU - Francis, Paul

AU - Sathaye, Sadhana

PY - 2018/8

Y1 - 2018/8

N2 - Objectives: Considering the deleterious effect of Aβ1-42, a study was designed to evaluate the effect of phloretin on altered synaptic proteins and adult hippocampal neurogenesis in Aβ1-42-injected Wistar rats. Methods: The rats were pretreated with 5 mg/kg p.o dose of phloretin and donepezil (positive control) for 28 days, followed by intrahippocampal injections of aggregated Aβ1-42. After termination, perfused brains were isolated and subjected to Western blot and immunohistochemistry (IHC) analysis. Key findings: The Western blot revealed that Aβ1-42-injected rats had significantly low levels of synaptophysin as compared to sham control. Phloretin pretreatment significantly protected the presynaptic protein synaptophysin against the effects of Aβ1-42. There were no significant changes in the levels of PSD95 between different groups. The IHC findings showed that Aβ1-42 significantly reduced the Ki67 and DCX in the dentate gyrus as compared to sham control. However, phloretin significantly improved the number of Ki67- and DCX-positive neurons in the dentate gyrus region as compared to Aβ1-42 group. Conclusions: This study demonstrated the protective effect of phloretin on synaptophysin and adult neuronal proliferating cells in Aβ1-42-injected rats. The encouraging findings highlight the potential of phloretin as a dietary supplement targeting key therapeutic mechanisms in neurodegenerative disorders such as AD.

AB - Objectives: Considering the deleterious effect of Aβ1-42, a study was designed to evaluate the effect of phloretin on altered synaptic proteins and adult hippocampal neurogenesis in Aβ1-42-injected Wistar rats. Methods: The rats were pretreated with 5 mg/kg p.o dose of phloretin and donepezil (positive control) for 28 days, followed by intrahippocampal injections of aggregated Aβ1-42. After termination, perfused brains were isolated and subjected to Western blot and immunohistochemistry (IHC) analysis. Key findings: The Western blot revealed that Aβ1-42-injected rats had significantly low levels of synaptophysin as compared to sham control. Phloretin pretreatment significantly protected the presynaptic protein synaptophysin against the effects of Aβ1-42. There were no significant changes in the levels of PSD95 between different groups. The IHC findings showed that Aβ1-42 significantly reduced the Ki67 and DCX in the dentate gyrus as compared to sham control. However, phloretin significantly improved the number of Ki67- and DCX-positive neurons in the dentate gyrus region as compared to Aβ1-42 group. Conclusions: This study demonstrated the protective effect of phloretin on synaptophysin and adult neuronal proliferating cells in Aβ1-42-injected rats. The encouraging findings highlight the potential of phloretin as a dietary supplement targeting key therapeutic mechanisms in neurodegenerative disorders such as AD.

KW - antioxidants

KW - flavonoids

KW - neurogenesis

KW - phloretin

KW - synaptophysin

UR - http://www.scopus.com/inward/record.url?scp=85045838131&partnerID=8YFLogxK

U2 - 10.1111/jphp.12925

DO - 10.1111/jphp.12925

M3 - Article

C2 - 29682750

AN - SCOPUS:85045838131

VL - 70

SP - 1022

EP - 1030

JO - Journal of Pharmacy and Pharmacology

JF - Journal of Pharmacy and Pharmacology

SN - 0022-3573

IS - 8

ER -

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