The effect of sports injury on insulin-like growth factor-I and type 3 procollagen: Implications for detection of growth hormone abuse in athletes

TY - JOUR

T1 - The effect of sports injury on insulin-like growth factor-I and type 3 procollagen: Implications for detection of growth hormone abuse in athletes

AU - Erotokritou-Mulligan, I

AU - Bassett, E E

AU - Bartlett, C

AU - Cowan, D

AU - McHugh, C

AU - Seah, R

AU - Curtis, B

AU - Wells, V

AU - Harrison, K

AU - Sonksen, P H

AU - Holt, R I G

PY - 2008/7

Y1 - 2008/7

N2 - Context: A method to detect exogenously administered growth hormone (GH) based on the measurement of two GH-dependent markers, IGF-I and type 3 procollagen (P-III-P) has been proposed. Skeletal or soft tissue injury may alter these markers. Elevations in either of these proteins after injury might lead to a false accusation of doping with GH. Objective: The objective of the study was to assess the effect of musculoskeletal or soft tissue injury on IGF-I and P-III-P concentrations in amateur and elite athletes and assess the effect of injury on the proposed GH detection method. Design: This was a longitudinal observational study after sporting injury. Setting: The study was conducted at Southampton General Hospital and British Olympic Medical Centre. Subjects: Subjects included elite and amateur athletes after an injury. Intervention: Interventions included measurement of IGF-I and P-III-P and application of the GH-2000 discriminant function score up to 84 d after an injury as well as classification of injury by type and severity. Outcome Measures: IGF-I and P-III-P concentration and ability to detect GH abuse in athletes without the risk of false accusation because of an injury were measured. Results: There was no change in IGF-I concentration after an injury. By contrast, P-III-P concentrations rose by 41.1 +/- 16.6%, reaching a peak around 14 d after an injury. The rise in P-III-P varied according to injury type and severity. This rise had a trivial effect on the GH-2000 discriminant function score, and no subject reached the threshold needed for a doping offense. Conclusions: Although there was a rise in P-III-P after injury, this was insufficient to invalidate the GH-2000 detection method based on IGF-I and P-III-P concentrations

AB - Context: A method to detect exogenously administered growth hormone (GH) based on the measurement of two GH-dependent markers, IGF-I and type 3 procollagen (P-III-P) has been proposed. Skeletal or soft tissue injury may alter these markers. Elevations in either of these proteins after injury might lead to a false accusation of doping with GH. Objective: The objective of the study was to assess the effect of musculoskeletal or soft tissue injury on IGF-I and P-III-P concentrations in amateur and elite athletes and assess the effect of injury on the proposed GH detection method. Design: This was a longitudinal observational study after sporting injury. Setting: The study was conducted at Southampton General Hospital and British Olympic Medical Centre. Subjects: Subjects included elite and amateur athletes after an injury. Intervention: Interventions included measurement of IGF-I and P-III-P and application of the GH-2000 discriminant function score up to 84 d after an injury as well as classification of injury by type and severity. Outcome Measures: IGF-I and P-III-P concentration and ability to detect GH abuse in athletes without the risk of false accusation because of an injury were measured. Results: There was no change in IGF-I concentration after an injury. By contrast, P-III-P concentrations rose by 41.1 +/- 16.6%, reaching a peak around 14 d after an injury. The rise in P-III-P varied according to injury type and severity. This rise had a trivial effect on the GH-2000 discriminant function score, and no subject reached the threshold needed for a doping offense. Conclusions: Although there was a rise in P-III-P after injury, this was insufficient to invalidate the GH-2000 detection method based on IGF-I and P-III-P concentrations

U2 - 10.1210/jc.2007-2801

DO - 10.1210/jc.2007-2801

M3 - Article

SN - 1945-7197

VL - 93

SP - 2760

EP - 2763

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

IS - 7

ER -