The first enzymatic method for C-F-18 bond formation: the synthesis of 5 '-[F-18]-fluoro-5 ' deoxyadenosine for imaging with PET

TY - JOUR

T1 - The first enzymatic method for C-F-18 bond formation: the synthesis of 5 '-[F-18]-fluoro-5 ' deoxyadenosine for imaging with PET

AU - Martarello, L

AU - Schaffrath, C

AU - Deng, H

AU - Gee, A D

AU - Lockhart, A

AU - O'Hagan, D

PY - 2003/11

Y1 - 2003/11

N2 - The use of the key enzyme involved in carbon-fluorine bond formation in Streptomyces cattleya catalysing the formation of 5'-fluoro-5'-deoxyadenosine (5'-FDA) from fluoride ion and S-adenosyl-L-methionine (SAM) was explored for its potential application in fluorine-18 labelling of the adenosine derivative. Enzymatic radiolabelling of [F-18]-5'-FDA was successfully carried out starting from SAM and [F-18]HF when the concentration of the enzyme preparation was increased from sub-mg/ml values to mg/ml values. The purity of the enzyme had no measurable effect on the radiochemical yield of the reaction and the radiochemical purity of [F-18]-5'-FDA.

AB - The use of the key enzyme involved in carbon-fluorine bond formation in Streptomyces cattleya catalysing the formation of 5'-fluoro-5'-deoxyadenosine (5'-FDA) from fluoride ion and S-adenosyl-L-methionine (SAM) was explored for its potential application in fluorine-18 labelling of the adenosine derivative. Enzymatic radiolabelling of [F-18]-5'-FDA was successfully carried out starting from SAM and [F-18]HF when the concentration of the enzyme preparation was increased from sub-mg/ml values to mg/ml values. The purity of the enzyme had no measurable effect on the radiochemical yield of the reaction and the radiochemical purity of [F-18]-5'-FDA.

U2 - 10.1002/jlcr.779

DO - 10.1002/jlcr.779

M3 - Article

SN - 0362-4803

VL - 46

SP - 1181

EP - 1189

JO - JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS

JF - JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS

IS - 13

ER -