The gene CYP3 encoding P450pcn1 (nifedipine oxidase) is tightly linked to the gene COL1A2 encoding collagen type 1 alpha on 7q21-q22.1

B A Brooks, O W McBride, C T Dolphin, M Farrall, P J Scambler, F J Gonzalez, J R Idle

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

CYP3, the gene which encodes the hepatic cytochrome P450pcn1, the isozyme responsible for the metabolic oxidation of the calcium channel-blocking drug nifedipine, has recently been mapped to human chromosome 7 using somatic cell hybrids. Using multilocus linkage analysis in CEPH families, we examined the linkage of a cDNA probe (hPCN1) for CYP3 to the oncogene MET, the pro-alpha 2(1) collagen gene COL1A2, and the T-cell receptor beta-chain gene TCRB, together with three arbitrary loci D7S8, D7S13, and D7S16, defined by the anonymous DNA probes pJ3.11, pB79a, and p7C22, respectively. From 70 CEPH parents screened with a StyI RFLP for hPCN1, four informative families were found each with both parental and maternal grandparents and 6-11 children per family. Tight linkage emerged between CYP3 and COL1A2, with a maximum combined lod score of 5.72 at theta = 0, suggesting the most likely subchromosomal localization of CYP3 is 7q21.3-q22.1.
Original languageEnglish
Pages (from-to)280-4
Number of pages5
JournalAmerican Journal of Human Genetics
Volume43
Issue number3
Publication statusPublished - Sept 1988

Keywords

  • Chromosomes, Human, Pair 7
  • Cloning, Molecular
  • Collagen
  • Cytochrome P-450 CYP3A
  • Cytochrome P-450 Enzyme System
  • Female
  • Genetic Linkage
  • Humans
  • Male
  • Mixed Function Oxygenases
  • Nifedipine
  • Pedigree
  • Polymorphism, Restriction Fragment Length

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