@article{34ef70279a984b36846814b3597ef5ea,
title = "The histone modification H3K4me3 is altered at the ANK1 locus in Alzheimer's disease brain",
abstract = "Several epigenome-wide association studies of DNA methylation have highlighted altered DNA methylation in the ANK1 gene in Alzheimer's disease (AD) brain samples. However, no study has specifically examined ANK1 histone modifications in the disease. We use chromatin immunoprecipitation-qPCR to quantify tri-methylation at histone 3 lysine 4 (H3K4me3) and 27 (H3K27me3) in the ANK1 gene in entorhinal cortex from donors with high (n = 59) or low (n = 29) Alzheimer's disease pathology. We demonstrate decreased levels of H3K4me3, a marker of active gene transcription, with no change in H3K27me3, a marker of inactive genes. H3K4me3 is negatively correlated with DNA methylation in specific regions of the ANK1 gene. Our study suggests that the ANK1 gene shows altered epigenetic marks indicative of reduced gene activation in Alzheimer's disease. ",
keywords = "Alzheimer's disease, ANK1, brain, chromatin modifications, DNA methylation, Epigenetics, H3K27me3, H3K4me3, histone modifications",
author = "Smith, {Adam R.} and Smith, {Rebecca G.} and Ruby Macdonald and Marzi, {Sarah J.} and Joe Burrage and Claire Troakes and Safa Al-Sarraj and Jonathan Mill and Katie Lunnon",
note = "Funding Information: This work was funded by a PhD studentship from BRACE (Bristol Research into Alzheimer{\textquoteright}s and Care of the Elderly) to K Lunnon, an Alzheimer{\textquoteright}s Society project grant (AS-PG-14-038) to K Lunnon, an Alzheimer{\textquoteright}s Association US New Investigator Research Grant (NIRG-14-320878) to K Lunnon, a pilot project grant to KL from Alzheimer{\textquoteright}s Research UK (ARUK-PPG2017B-021), a project grant from the Medical Research Council (MRC) (MR/N027973/1) to K Lunnon as part of the Joint Programme – Neurodegenerative Disease Research (JPND) initiative and a NIH R01 grant AG036039 to J Mill. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Funding Information: The authors thank the Brains for Dementia Research Initiative (jointly funded by the Alzheimer{\textquoteright}s Society and Alzheimer{\textquoteright}s Research UK), the UK Brain Bank Network (UKBBN) and the donors and families who made this research possible. Publisher Copyright: {\textcopyright} 2021 Katie Lunnon.",
year = "2021",
month = apr,
day = "1",
doi = "10.2144/FSOA-2020-0161",
language = "English",
volume = "7",
journal = "Future Science OA",
issn = "2056-5623",
publisher = "Future Medicine Ltd.",
number = "4",
}