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The Landscape of Coding and Noncoding RNAs in Platelets

Research output: Contribution to journalReview articlepeer-review

Original languageEnglish
Pages (from-to)1200-1216
Number of pages17
JournalAntioxidants and Redox Signaling
Volume34
Issue number15
DOIs
Published20 May 2021

Bibliographical note

Funding Information: C.G. is funded by a British Heart Foundation (BHF) PhD studentship (FS/18/60/34181). A.J. was a British Heart Foundation Clinical Research Training Fellow (FS/16/32/ 32184). M.M. is a BHF Chair Holder (CH/16/3/32406) with BHF program grant support (RG/16/14/32397) and member of a network on ‘‘MicroRNA-based Therapeutic Strategies in Vascular Disease’’ funded by the Foundation Leducq. This study is also supported by VASCage—Research Centre on Vascular Ageing and Stroke. As a COMET centre, VASCage is funded within the COMET program—Competence Centers for Excellent Technologies by the Austrian Ministry for Climate Action, Environment, Energy, Mobility, Innovation and Technology; the Austrian Ministry for Digital and Economic Affairs; and the federal states Tyrol, Salzburg, and Vienna. Publisher Copyright: © 2021 BMJ Publishing Group. All rights reserved. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

King's Authors

Abstract

Significance: Levels of platelet noncoding RNAs (ncRNAs) are altered by disease, and ncRNAs may exert functions inside and outside of platelets. Their role in physiologic hemostasis and pathologic thrombosis remains to be explored. Recent Advances: The number of RNA classes identified in platelets has been growing since the past decade. Apart from coding messenger RNAs, the RNA landscape in platelets comprises ncRNAs such as microRNAs, circular RNAs, long ncRNAs, YRNAs, and potentially environmentally derived exogenous ncRNAs. Recent research has focused on the function of platelet RNAs beyond platelets, mediated through protective RNA shuttles or even cellular uptake of entire platelets. Multiple studies have also explored the potential of platelet RNAs as novel biomarkers. Critical Issues: Platelet preparations can contain contaminating leukocytes. Even few leukocytes may contribute a substantial amount of RNA. As biomarkers, platelet RNAs have shown associations with platelet activation, but it remains to be seen whether their measurements could improve diagnostics. It also needs to be clarified whether platelet RNAs influence processes beyond platelets. Future Directions: Technological advances such as single-cell RNA-sequencing might help to identify hyperreactive platelet subpopulations on a single-platelet level, avoid the common problem of leukocyte contamination in platelet preparations, and allow simultaneous profiling of native megakaryocytes and their platelet progeny to clarify to what extent the platelet RNA content reflects their megakaryocyte precursors or changes in the circulation.

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