The Localization of the Integral Membrane Protein Cps1p to the Cell Division Site is Dependent on the Actomyosin Ring and the Septation-Inducing Network in Schizosaccharomyces pombe

Jianhua Liu, Xie Tang, Hongyan Wang, Snezhana Oliferenko, Mohan K Balasubramanian

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)

Abstract

Schizosaccharomyces pombe cells divide by medial fission through the use of an actomyosin-based contractile ring. Constriction of the actomyosin ring is accompanied by the centripetal addition of new membranes and cell wall material. In this article, we characterize the mechanism responsible for the localization of Cps1p, a septum-synthesizing 1,3-beta-glucan synthase, to the division site during cytokinesis. We show that Cps1p is an integral membrane protein that localizes to the cell division site late in anaphase. Neither F-actin nor microtubules are essential for the initial assembly of Cps1p to the medial division site. F-actin, but not microtubules, is however important for the eventual incorporation of Cps1p into the actomyosin ring. Assembly of Cps1p into the cell division ring is also dependent on the septation-inducing network (SIN) proteins that regulate division septum formation after assembly of the actomyosin ring. Fluorescence-recovery after-photobleaching experiments reveal that Cps1p does not diffuse appreciably within the plasma membrane and is retained at the division site by a mechanism that does not depend on an intact F-actin cytoskeleton. We conclude that the actomyosin ring serves as a spatial cue for Cps1p localization, whereas the maintenance of Cps1p at the division site occurs by a novel F-actin- and microtubule-independent mechanism. Furthermore, we propose that the SIN proteins ensure localization of Cps1p at the appropriate point in the cell cycle.

Original languageEnglish
Pages (from-to)989-1000
Number of pages12
JournalMolecular biology of the cell
Volume13
Issue number3
DOIs
Publication statusPublished - 1 Mar 2002

Keywords

  • Actins
  • Brefeldin A
  • Cell Division
  • DNA
  • Ethanol
  • Glucosyltransferases
  • Green Fluorescent Proteins
  • Indicators and Reagents
  • Luminescent Proteins
  • Membrane Proteins
  • Microtubules
  • Protein Synthesis Inhibitors
  • Recombinant Fusion Proteins
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins

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