The multi-mode polarization modulation spectrometer: part 1: simultaneous detection of absorption, turbidity, and optical activity

T Arvinte; T T T Bui; A A Dahab; B Demeule; A F Drake; D Elhag; P King

doi:10.1016/j.ab.2004.05.045

The multi-mode polarization modulation spectrometer: part 1: simultaneous detection of absorption, turbidity, and optical activity

T Arvinte, T T T Bui, A A Dahab, B Demeule, A F Drake, D Elhag, P King

Institute of Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

Circular dichroism (CD) is an important spectroscopic technique for monitoring chirality and biological macromolecule conformation. However, during a CD measurement, absorbance, light scattering/turbidity, and fluorescence can also be detected. The simultaneous measurement of these different spectral features for a single sample is the basis of a multi-mode optical spectrometer. This allows time-efficient gathering of complementary information and provides a scheme to ensure that CD measurements are reliable. Aspects of circular polarization differential light scattering, pH, and temperature variation of a protein (antibody) solution are described. A procedure to help ensure that CD measurements are reliable is described. (C) 2004 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	46 - 57
Number of pages	12
Journal	Analytical Biochemistry
Volume	332
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2004.05.045
Publication status	Published - 1 Sept 2004

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abstract = "Circular dichroism (CD) is an important spectroscopic technique for monitoring chirality and biological macromolecule conformation. However, during a CD measurement, absorbance, light scattering/turbidity, and fluorescence can also be detected. The simultaneous measurement of these different spectral features for a single sample is the basis of a multi-mode optical spectrometer. This allows time-efficient gathering of complementary information and provides a scheme to ensure that CD measurements are reliable. Aspects of circular polarization differential light scattering, pH, and temperature variation of a protein (antibody) solution are described. A procedure to help ensure that CD measurements are reliable is described. (C) 2004 Elsevier Inc. All rights reserved.",

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AU - Arvinte, T

AU - Bui, T T T

AU - Dahab, A A

AU - Demeule, B

AU - Drake, A F

AU - Elhag, D

AU - King, P

PY - 2004/9/1

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AB - Circular dichroism (CD) is an important spectroscopic technique for monitoring chirality and biological macromolecule conformation. However, during a CD measurement, absorbance, light scattering/turbidity, and fluorescence can also be detected. The simultaneous measurement of these different spectral features for a single sample is the basis of a multi-mode optical spectrometer. This allows time-efficient gathering of complementary information and provides a scheme to ensure that CD measurements are reliable. Aspects of circular polarization differential light scattering, pH, and temperature variation of a protein (antibody) solution are described. A procedure to help ensure that CD measurements are reliable is described. (C) 2004 Elsevier Inc. All rights reserved.

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

The multi-mode polarization modulation spectrometer: part 1: simultaneous detection of absorption, turbidity, and optical activity

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