The precision of textural analysis in 18F-FDG-PET scans of oesophageal cancer

Georgia Doumou, Musib Siddique, Charalampos Tsoumpas, Vicky Goh, Gary J. Cook*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)



Measuring tumour heterogeneity by textural analysis in 18F-fluorodeoxyglucose positron emission tomography (18F-FDG PET) provides predictive and prognostic information but technical aspects of image processing can influence parameter measurements. We therefore tested effects of image smoothing, segmentation and quantisation on the precision of heterogeneity measurements.


Sixty-four 18F-FDG PET/CT images of oesophageal cancer were processed using different Gaussian smoothing levels (2.0, 2.5, 3.0, 3.5, 4.0 mm), maximum standardised uptake value (SUVmax) segmentation thresholds (45 %, 50 %, 55 %, 60 %) and quantisation (8, 16, 32, 64, 128 bin widths). Heterogeneity parameters included grey-level co-occurrence matrix (GLCM), grey-level run length matrix (GLRL), neighbourhood grey-tone difference matrix (NGTDM), grey-level size zone matrix (GLSZM) and fractal analysis methods. The concordance correlation coefficient (CCC) for the three processing variables was calculated for each heterogeneity parameter.


Most parameters showed poor agreement between different bin widths (CCC median 0.08, range 0.004–0.99). Segmentation and smoothing showed smaller effects on precision (segmentation: CCC median 0.82, range 0.33–0.97; smoothing: CCC median 0.99, range 0.58–0.99).


Smoothing and segmentation have only a small effect on the precision of heterogeneity measurements in 18F-FDG PET data. However, quantisation often has larger effects, highlighting a need for further evaluation and standardisation of parameters for multicentre studies.
Original languageEnglish
Pages (from-to)2805-2812
Number of pages8
JournalEuropean Radiology
Issue number9
Early online date21 May 2015
Publication statusPublished - Sept 2015


  • <sup>18</sup>F-FDG PET
  • Heterogeneity
  • Oesophageal cancer
  • Precision
  • Texture analysis


Dive into the research topics of 'The precision of textural analysis in 18F-FDG-PET scans of oesophageal cancer'. Together they form a unique fingerprint.

Cite this