The relative efficiency of acquisition of MHC : peptide complexes and cross-presentation depends on dendritic cell type

Lesley Ann Smyth, Nicola Harker, Wayne Turnbull, Haytham El-Doueik, Linda Klavinskis, Dimitris Kioussis, Giovanna Lombardi, Robert Lechler

Research output: Contribution to journalArticlepeer-review

Abstract

Intercellular exchange of MHC molecules has been reported between many cells, including professional and nonprofessional APCs. This phenomenon may contribute to T cell immunity to pathogens. In this study, we addressed whether the transfer of MHC class I:peptide complexes between cells plays a role in T cell responses and compare this to conventional cross-presentation. We observed that dsRNA-matured bone marrow-derived dendritic cells (BMDCs) acquired peptide:MHC complexes from other BMDCs either pulsed with OVA(257-264) peptide, soluble OVA, or infected with a recombinant adenovirus expressing OVA. In addition, BMDCs were capable of acquiring MHC:peptide complexes from epithelial cells. Spleen-derived CD8 alpha(+) and CD8 alpha(-) dendritic cells (DCs) also acquired MHC:peptide complexes from BMDCs pulsed with OVA(257-264) peptide. However, the efficiency of acquisition by these ex vivo derived DCs is much lower than acquisition by BMDC. In all cases, the acquired MHC:peptide complexes were functional in that they induced Ag-specific CD8(+) T cell proliferation. The efficiency of MHC transfer was compared with cross-presentation for splenic CD8 alpha(+) and CD8 alpha(-) as well as BMDCs. CD8 alpha(-) DCs were more efficient at inducing T cell proliferation when they acquired Ag via cross-presentation, the opposite was observed for BMDCs and splenic CD8 alpha(-) DCs. We conclude from these observations that the relative efficiency of MHC transfer vs cross-presentation differs markedly between different DC subsets.
Original languageEnglish
Pages (from-to)3212 - 3220
Number of pages9
JournalJournal of Immunology
Volume181
Issue number5
Publication statusPublished - 2008

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