The relative role of peripheral blood and bone marrow for monitoring molecular evidence of disease in follicular lymphoma by quantitative real-time polymerase chain reaction

KE Summers; AJ Davies; J Matthews; MJ Jenner; V Cornelius; JA Amess; AJ Norton; AZS Rohatiner; J Fitzgibbon; TA Lister; LK Goff

doi:10.1046/j.1365-2141.2002.03641.x

The relative role of peripheral blood and bone marrow for monitoring molecular evidence of disease in follicular lymphoma by quantitative real-time polymerase chain reaction

KE Summers
, AJ Davies
, J Matthews
, MJ Jenner
, V Cornelius
, JA Amess
, AJ Norton
, AZS Rohatiner
, J Fitzgibbon
, TA Lister
, LK Goff^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Peripheral blood (PB) and bone marrow (BM) are used interchangeably for t(14;18) (IgH/BCL-2) molecular monitoring in follicular lymphoma (FL) and detection of rearrangement after treatment has been correlated to increased risk of relapse. To determine the relative value of each tissue, MBR t(14;18) was quantified by real-time polymerase chain reaction in 52 simultaneous paired PB and BM samples from 38 FL patients. In total, 79% of sample pairs taken in remission (n = 19) or when no morphological disease was evident in the BM (n = 29) had t(14;18) copy number within one log difference and the median difference was small. These findings suggest that, in remission, PB may be adequately monitored. In general, however, higher copy number was detected in BM than in the corresponding PB sample.

Original language	English
Pages (from-to)	563-566
Number of pages	4
Journal	British Journal of Haematology
Volume	118
Issue number	2
DOIs	https://doi.org/10.1046/j.1365-2141.2002.03641.x
Publication status	Published - Aug 2002

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

t(14;18)
follicular lymphoma
real-time PCR
monitoring
quantification
CELLS
TRANSLOCATION
REARRANGEMENT
RELAPSE

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10.1046/j.1365-2141.2002.03641.x

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Summers, KE., Davies, AJ., Matthews, J., Jenner, MJ., Cornelius, V., Amess, JA., Norton, AJ., Rohatiner, AZS., Fitzgibbon, J., Lister, TA., & Goff, LK. (2002). The relative role of peripheral blood and bone marrow for monitoring molecular evidence of disease in follicular lymphoma by quantitative real-time polymerase chain reaction. British Journal of Haematology, 118(2), 563-566. https://doi.org/10.1046/j.1365-2141.2002.03641.x

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title = "The relative role of peripheral blood and bone marrow for monitoring molecular evidence of disease in follicular lymphoma by quantitative real-time polymerase chain reaction",

abstract = "Peripheral blood (PB) and bone marrow (BM) are used interchangeably for t(14;18) (IgH/BCL-2) molecular monitoring in follicular lymphoma (FL) and detection of rearrangement after treatment has been correlated to increased risk of relapse. To determine the relative value of each tissue, MBR t(14;18) was quantified by real-time polymerase chain reaction in 52 simultaneous paired PB and BM samples from 38 FL patients. In total, 79\% of sample pairs taken in remission (n = 19) or when no morphological disease was evident in the BM (n = 29) had t(14;18) copy number within one log difference and the median difference was small. These findings suggest that, in remission, PB may be adequately monitored. In general, however, higher copy number was detected in BM than in the corresponding PB sample.",

keywords = "t(14;18), follicular lymphoma, real-time PCR, monitoring, quantification, CELLS, TRANSLOCATION, REARRANGEMENT, RELAPSE",

author = "KE Summers and AJ Davies and J Matthews and MJ Jenner and V Cornelius and JA Amess and AJ Norton and AZS Rohatiner and J Fitzgibbon and TA Lister and LK Goff",

year = "2002",

month = aug,

doi = "10.1046/j.1365-2141.2002.03641.x",

language = "English",

volume = "118",

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Summers, KE, Davies, AJ, Matthews, J, Jenner, MJ, Cornelius, V, Amess, JA, Norton, AJ, Rohatiner, AZS, Fitzgibbon, J, Lister, TA & Goff, LK 2002, 'The relative role of peripheral blood and bone marrow for monitoring molecular evidence of disease in follicular lymphoma by quantitative real-time polymerase chain reaction', British Journal of Haematology, vol. 118, no. 2, pp. 563-566. https://doi.org/10.1046/j.1365-2141.2002.03641.x

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T1 - The relative role of peripheral blood and bone marrow for monitoring molecular evidence of disease in follicular lymphoma by quantitative real-time polymerase chain reaction

AU - Summers, KE

AU - Davies, AJ

AU - Matthews, J

AU - Jenner, MJ

AU - Cornelius, V

AU - Amess, JA

AU - Norton, AJ

AU - Rohatiner, AZS

AU - Fitzgibbon, J

AU - Lister, TA

AU - Goff, LK

PY - 2002/8

Y1 - 2002/8

N2 - Peripheral blood (PB) and bone marrow (BM) are used interchangeably for t(14;18) (IgH/BCL-2) molecular monitoring in follicular lymphoma (FL) and detection of rearrangement after treatment has been correlated to increased risk of relapse. To determine the relative value of each tissue, MBR t(14;18) was quantified by real-time polymerase chain reaction in 52 simultaneous paired PB and BM samples from 38 FL patients. In total, 79% of sample pairs taken in remission (n = 19) or when no morphological disease was evident in the BM (n = 29) had t(14;18) copy number within one log difference and the median difference was small. These findings suggest that, in remission, PB may be adequately monitored. In general, however, higher copy number was detected in BM than in the corresponding PB sample.

AB - Peripheral blood (PB) and bone marrow (BM) are used interchangeably for t(14;18) (IgH/BCL-2) molecular monitoring in follicular lymphoma (FL) and detection of rearrangement after treatment has been correlated to increased risk of relapse. To determine the relative value of each tissue, MBR t(14;18) was quantified by real-time polymerase chain reaction in 52 simultaneous paired PB and BM samples from 38 FL patients. In total, 79% of sample pairs taken in remission (n = 19) or when no morphological disease was evident in the BM (n = 29) had t(14;18) copy number within one log difference and the median difference was small. These findings suggest that, in remission, PB may be adequately monitored. In general, however, higher copy number was detected in BM than in the corresponding PB sample.

KW - t(14;18)

KW - follicular lymphoma

KW - real-time PCR

KW - monitoring

KW - quantification

KW - CELLS

KW - TRANSLOCATION

KW - REARRANGEMENT

KW - RELAPSE

U2 - 10.1046/j.1365-2141.2002.03641.x

DO - 10.1046/j.1365-2141.2002.03641.x

M3 - Article

C2 - 12139746

SN - 0007-1048

VL - 118

SP - 563

EP - 566

JO - British Journal of Haematology

JF - British Journal of Haematology

IS - 2

ER -

The relative role of peripheral blood and bone marrow for monitoring molecular evidence of disease in follicular lymphoma by quantitative real-time polymerase chain reaction

Abstract

UN SDGs

Keywords

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