The retinoic acid metabolising gene, CYP26B1, patterns the cartilaginous cranial neural crest in zebrafish

S Reijntjes, A Rodaway, M Maden

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

We have investigated the function of the retinoic acid metabolising enzyme, CYP26131, by administering an antisense morpholino oligonucleotide to zebrafish embryos. The result was an alteration in the morphology of the embryo in those regions which express the gene, namely an embryo with a smaller head, correspondingly smaller hindbrain rhombomeres and severely reduced numbers of vagal brachiornotor neurons. Most strikingly, these embryos had defective or absent jaw cartilages implying a role for this enzyme in patterning or migration of the neural crest cells which give rise to this tissue type. In order to determine whether this phenotype resemblesthat of excess retinoic acid or a deficiency of retinoic acid, we compared the jaw defects following retinoic acid treatment or DEAB treatment, the latter being an inhibitor of retinoic acid synthesis. The effects of the inhibitor rather than excess retinoic acid most closely phenocopied the jaw defects seen with the Cyp26B1 morpholino which suggests that, at least in the zebrafish embryo, the action of CYP26131 in the neural crest may not be simply to catabolise all-trans-RA
Original languageEnglish
Pages (from-to)351 - 360
Number of pages10
JournalInternational Journal of Developmental Biology
Volume51
Issue number5
DOIs
Publication statusPublished - 2007

Fingerprint

Dive into the research topics of 'The retinoic acid metabolising gene, CYP26B1, patterns the cartilaginous cranial neural crest in zebrafish'. Together they form a unique fingerprint.

Cite this