The RNA-methyltransferase Misu (NSun2) poises epidermal stem cells to differentiate

Sandra Blanco; Agata Kurowski; Jennifer Nichols; Fiona M Watt; Salvador Aznar Benitah; Michaela Frye

doi:10.1371/journal.pgen.1002403

The RNA-methyltransferase Misu (NSun2) poises epidermal stem cells to differentiate

Sandra Blanco, Agata Kurowski, Jennifer Nichols, Fiona M Watt, Salvador Aznar Benitah, Michaela Frye

Gene Therapy and Regenerative Medicine

University of Cambridge

Research output: Contribution to journal › Article › peer-review

166 Citations (Scopus)

Abstract

Homeostasis of most adult tissues is maintained by balancing stem cell self-renewal and differentiation, but whether post-transcriptional mechanisms can regulate this process is unknown. Here, we identify that an RNA methyltransferase (Misu/Nsun2) is required to balance stem cell self-renewal and differentiation in skin. In the epidermis, this methyltransferase is found in a defined sub-population of hair follicle stem cells poised to undergo lineage commitment, and its depletion results in enhanced quiescence and aberrant stem cell differentiation. Our results reveal that post-transcriptional RNA methylation can play a previously unappreciated role in controlling stem cell fate.

Original language	English
Article number	e1002403
Pages (from-to)	N/A
Number of pages	14
Journal	PL o S Genetics
Volume	7
Issue number	12
DOIs	https://doi.org/10.1371/journal.pgen.1002403
Publication status	Published - Dec 2011

Keywords

Animals
Body Weight
Cell Differentiation
Embryonic Development
Epidermis
Hair Follicle
Homeostasis
Keratinocytes
Lymphoid Enhancer-Binding Factor 1
Methylation
Methyltransferases
Mice
Mice, Transgenic
RNA Processing, Post-Transcriptional
RNA, Transfer
Stem Cells
beta Catenin

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title = "The RNA-methyltransferase Misu (NSun2) poises epidermal stem cells to differentiate",

abstract = "Homeostasis of most adult tissues is maintained by balancing stem cell self-renewal and differentiation, but whether post-transcriptional mechanisms can regulate this process is unknown. Here, we identify that an RNA methyltransferase (Misu/Nsun2) is required to balance stem cell self-renewal and differentiation in skin. In the epidermis, this methyltransferase is found in a defined sub-population of hair follicle stem cells poised to undergo lineage commitment, and its depletion results in enhanced quiescence and aberrant stem cell differentiation. Our results reveal that post-transcriptional RNA methylation can play a previously unappreciated role in controlling stem cell fate.",

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AU - Kurowski, Agata

AU - Nichols, Jennifer

AU - Watt, Fiona M

AU - Benitah, Salvador Aznar

AU - Frye, Michaela

PY - 2011/12

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AB - Homeostasis of most adult tissues is maintained by balancing stem cell self-renewal and differentiation, but whether post-transcriptional mechanisms can regulate this process is unknown. Here, we identify that an RNA methyltransferase (Misu/Nsun2) is required to balance stem cell self-renewal and differentiation in skin. In the epidermis, this methyltransferase is found in a defined sub-population of hair follicle stem cells poised to undergo lineage commitment, and its depletion results in enhanced quiescence and aberrant stem cell differentiation. Our results reveal that post-transcriptional RNA methylation can play a previously unappreciated role in controlling stem cell fate.

KW - Animals

KW - Body Weight

KW - Cell Differentiation

KW - Embryonic Development

KW - Epidermis

KW - Hair Follicle

KW - Homeostasis

KW - Keratinocytes

KW - Lymphoid Enhancer-Binding Factor 1

KW - Methylation

KW - Methyltransferases

KW - Mice

KW - Mice, Transgenic

KW - RNA Processing, Post-Transcriptional

KW - RNA, Transfer

KW - Stem Cells

KW - beta Catenin

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DO - 10.1371/journal.pgen.1002403

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The RNA-methyltransferase Misu (NSun2) poises epidermal stem cells to differentiate

Abstract

Keywords

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