The role of intrinsic disorder and dynamics in the assembly and function of the type II secretion system

Shuang Gu; Vladimir E. Shevchik; Rosie Shaw; Richard W. Pickersgill; James A. Garnett

doi:10.1016/j.bbapap.2017.07.006

The role of intrinsic disorder and dynamics in the assembly and function of the type II secretion system

Shuang Gu, Vladimir E. Shevchik, Rosie Shaw, Richard W. Pickersgill^*, James A. Garnett

^*Corresponding author for this work

Centre for Host Microbiome Interactions

Research output: Contribution to journal › Review article › peer-review

26 Citations (Scopus)

Abstract

Many Gram-negative commensal and pathogenic bacteria use a type II secretion system (T2SS) to transport proteins out of the cell. These exported proteins or substrates play a major role in toxin delivery, maintaining biofilms, replication in the host and subversion of host immune responses to infection. We review the current structural and functional work on this system and argue that intrinsically disordered regions and protein dynamics are central for assembly, exo-protein recognition, and secretion competence of the T2SS. The central role of intrinsic disorder-order transitions in these processes may be a particular feature of type II secretion.

Original language	English
Pages (from-to)	1255-1266
Number of pages	12
Journal	Biochimica et Biophysica Acta - Proteins and Proteomics
Volume	1865
Issue number	10
DOIs	https://doi.org/10.1016/j.bbapap.2017.07.006
Publication status	Published - 1 Oct 2017

Keywords

Dynamics
Gram negative bacteria
Intrinsic disorder
Pathogen
Secretin
Secretion
Type II secretion system
Type III secretion system
Type IV pilus
Virulence

Access to Document

10.1016/j.bbapap.2017.07.006

Cite this

@article{97ba3e80d8ad493dbca2ef529c79ad10,

title = "The role of intrinsic disorder and dynamics in the assembly and function of the type II secretion system",

abstract = "Many Gram-negative commensal and pathogenic bacteria use a type II secretion system (T2SS) to transport proteins out of the cell. These exported proteins or substrates play a major role in toxin delivery, maintaining biofilms, replication in the host and subversion of host immune responses to infection. We review the current structural and functional work on this system and argue that intrinsically disordered regions and protein dynamics are central for assembly, exo-protein recognition, and secretion competence of the T2SS. The central role of intrinsic disorder-order transitions in these processes may be a particular feature of type II secretion.",

keywords = "Dynamics, Gram negative bacteria, Intrinsic disorder, Pathogen, Secretin, Secretion, Type II secretion system, Type III secretion system, Type IV pilus, Virulence",

author = "Shuang Gu and Shevchik, {Vladimir E.} and Rosie Shaw and Pickersgill, {Richard W.} and Garnett, {James A.}",

year = "2017",

month = oct,

day = "1",

doi = "10.1016/j.bbapap.2017.07.006",

language = "English",

volume = "1865",

pages = "1255--1266",

journal = "Biochimica et Biophysica Acta - Proteins and Proteomics",

issn = "1570-9639",

publisher = "Elsevier",

number = "10",

}

TY - JOUR

T1 - The role of intrinsic disorder and dynamics in the assembly and function of the type II secretion system

AU - Gu, Shuang

AU - Shevchik, Vladimir E.

AU - Shaw, Rosie

AU - Pickersgill, Richard W.

AU - Garnett, James A.

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Many Gram-negative commensal and pathogenic bacteria use a type II secretion system (T2SS) to transport proteins out of the cell. These exported proteins or substrates play a major role in toxin delivery, maintaining biofilms, replication in the host and subversion of host immune responses to infection. We review the current structural and functional work on this system and argue that intrinsically disordered regions and protein dynamics are central for assembly, exo-protein recognition, and secretion competence of the T2SS. The central role of intrinsic disorder-order transitions in these processes may be a particular feature of type II secretion.

AB - Many Gram-negative commensal and pathogenic bacteria use a type II secretion system (T2SS) to transport proteins out of the cell. These exported proteins or substrates play a major role in toxin delivery, maintaining biofilms, replication in the host and subversion of host immune responses to infection. We review the current structural and functional work on this system and argue that intrinsically disordered regions and protein dynamics are central for assembly, exo-protein recognition, and secretion competence of the T2SS. The central role of intrinsic disorder-order transitions in these processes may be a particular feature of type II secretion.

KW - Dynamics

KW - Gram negative bacteria

KW - Intrinsic disorder

KW - Pathogen

KW - Secretin

KW - Secretion

KW - Type II secretion system

KW - Type III secretion system

KW - Type IV pilus

KW - Virulence

UR - http://www.scopus.com/inward/record.url?scp=85027567165&partnerID=8YFLogxK

U2 - 10.1016/j.bbapap.2017.07.006

DO - 10.1016/j.bbapap.2017.07.006

M3 - Review article

C2 - 28733198

AN - SCOPUS:85027567165

SN - 1570-9639

VL - 1865

SP - 1255

EP - 1266

JO - Biochimica et Biophysica Acta - Proteins and Proteomics

JF - Biochimica et Biophysica Acta - Proteins and Proteomics

IS - 10

ER -

The role of intrinsic disorder and dynamics in the assembly and function of the type II secretion system

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this