@inbook{25c2217dec8645dea6fd8720089ada5d,
title = "The study of polycyclic aromatic hydrocarbon bioavailability in soils",
abstract = "In this study the efficacy of biological methods for measuring the bioavailability of PAHs was examined by means of luminescent biosensors and C-14-PAH mineralisation. The lux-tagged biosensor Pseudomonas fluorescens HK44 was able to grow on naphthalene as a sole carbon source. Bioluminescence was induced only by naphthalene. The naphthalene degradation kinetics and substrate specificity of P. fluorescens HK44 were investigated further by means of 14 C-naphthalene mineralisation assays. A Michaelis-Menten naphthalene K, value of 45 muM was derived for P. fluorescens HK44. Naphthalene bioavailability was assessed in the presence of a well-characterised un-contaminated sandy loam soil. Over a period of 1 hour incubation with added culture the mineralisation of added naphthalene was reduced by 30%. Further sorption of naphthalene in the presence of un-inoculated soil for 1 week resulted in an 80% reduction of the initial rate Of CO2 generation by comparison with a non-soil control. In addition the total stoichiometric amount Of CO2 evolved over a longer assay period was also substantially reduced upon sorption. These data may be interpreted in terms of a considerable reduction in naphthalene bioavailability.",
author = "Magar, {V S} and G Johnson and Ong, {S K} and A Leeson",
year = "2001",
language = "English",
isbn = "1-57477-113-2",
series = "BIOREMEDIATION SERIES",
publisher = "BATTELLE PRESS",
pages = "173 -- 180",
booktitle = "BIOREMED SER",
note = "6th International In Situ and On-Site Bioremediation Symposium ; Conference date: 01-01-2001",
}