The study of polycyclic aromatic hydrocarbon bioavailability in soils

V S Magar (Editor), G Johnson, S K Ong (Editor), A Leeson

Research output: Chapter in Book/Report/Conference proceedingConference paper

Abstract

In this study the efficacy of biological methods for measuring the bioavailability of PAHs was examined by means of luminescent biosensors and C-14-PAH mineralisation. The lux-tagged biosensor Pseudomonas fluorescens HK44 was able to grow on naphthalene as a sole carbon source. Bioluminescence was induced only by naphthalene. The naphthalene degradation kinetics and substrate specificity of P. fluorescens HK44 were investigated further by means of 14 C-naphthalene mineralisation assays. A Michaelis-Menten naphthalene K, value of 45 muM was derived for P. fluorescens HK44. Naphthalene bioavailability was assessed in the presence of a well-characterised un-contaminated sandy loam soil. Over a period of 1 hour incubation with added culture the mineralisation of added naphthalene was reduced by 30%. Further sorption of naphthalene in the presence of un-inoculated soil for 1 week resulted in an 80% reduction of the initial rate Of CO2 generation by comparison with a non-soil control. In addition the total stoichiometric amount Of CO2 evolved over a longer assay period was also substantially reduced upon sorption. These data may be interpreted in terms of a considerable reduction in naphthalene bioavailability.
Original languageEnglish
Title of host publicationBIOREMED SER
Place of PublicationCOLUMBUS
PublisherBATTELLE PRESS
Pages173 - 180
Number of pages8
ISBN (Print)1-57477-113-2
Publication statusPublished - 2001
Event6th International In Situ and On-Site Bioremediation Symposium - SAN DIEGO, CALIFORNIA
Duration: 1 Jan 2001 → …

Publication series

NameBIOREMEDIATION SERIES

Conference

Conference6th International In Situ and On-Site Bioremediation Symposium
CitySAN DIEGO, CALIFORNIA
Period1/01/2001 → …

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