Three-dimensional super-resolution fluorescence imaging of DNA

Sevim Yardimci, Daniel Burnham, Samantha Terry, Hasan Yardimci

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Abstract

Recent advances in fluorescence super-resolution microscopy are providing important insights into details of cellular structures. To acquire three dimensional (3D) superresolution images of DNA, we combined binding activated localization microscopy (BALM) using fluorescent double-stranded DNA intercalators and optical astigmatism. We quantitatively establish the advantage of bis- over mono-intercalators before demonstrating the approach by visualizing single DNA molecules stretched between microspheres at various heights. Finally, the approach is applied to the more complex environment of intact and damaged metaphase chromosomes,unravelling their structural features.
Original languageEnglish
JournalScientific Reports
Publication statusAccepted/In press - 2 Jul 2020

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