Time-domain fluorescence lifetime imaging techniques suitable for solid-state imaging sensor arrays

D.D.-U. Li, Simon Ameer-Beg, J. Arlt, D.d Tyndall, R.d Walker, D.R.e Matthews, Viput Visitkul, J.d Richardson, R.K.d Henderson

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

We have successfully demonstrated video-rate CMOS single-photon avalanche diode (SPAD)-based cameras for fluorescence lifetime imaging microscopy (FLIM) by applying innovative FLIM algorithms. We also review and compare several time-domain techniques and solid-state FLIM systems, and adapt the proposed algorithms for massive CMOS SPAD-based arrays and hardware implementations. The theoretical error equations are derived and their performances are demonstrated on the data obtained from 0.13 μm CMOS SPAD arrays and the multiple-decay data obtained from scanning PMT systems. In vivo two photon fluorescence lifetime imaging data of FITC-albumin labeled vasculature of a P22 rat carcinosarcoma (BD9 rat window chamber) are used to test how different algorithms perform on bi-decay data. The proposed techniques are capable of producing lifetime images with enough contrast.
Original languageEnglish
Pages (from-to)5650-5669
Number of pages20
JournalSensors (Switzerland)
Volume12
Issue number5
DOIs
Publication statusPublished - 2012

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