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Time-lapse, photoactivation, and photobleaching imaging of nucleolar assembly after mitosis

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Time-lapse, photoactivation, and photobleaching imaging of nucleolar assembly after mitosis. / Hernandez-Verdun, Danièle; Louvet, Emilie; Muro, Eleonora.

Imaging Gene Expression: Methods and Protocols. ed. / Yaron Shav-Tal. Humana Press, 2013. p. 337-350 (Methods in Molecular Biology; Vol. 1042).

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Harvard

Hernandez-Verdun, D, Louvet, E & Muro, E 2013, Time-lapse, photoactivation, and photobleaching imaging of nucleolar assembly after mitosis. in Y Shav-Tal (ed.), Imaging Gene Expression: Methods and Protocols. Methods in Molecular Biology, vol. 1042, Humana Press, pp. 337-350. https://doi.org/10.1007/978-1-62703-526-2_22

APA

Hernandez-Verdun, D., Louvet, E., & Muro, E. (2013). Time-lapse, photoactivation, and photobleaching imaging of nucleolar assembly after mitosis. In Y. Shav-Tal (Ed.), Imaging Gene Expression: Methods and Protocols (pp. 337-350). (Methods in Molecular Biology; Vol. 1042). Humana Press. https://doi.org/10.1007/978-1-62703-526-2_22

Vancouver

Hernandez-Verdun D, Louvet E, Muro E. Time-lapse, photoactivation, and photobleaching imaging of nucleolar assembly after mitosis. In Shav-Tal Y, editor, Imaging Gene Expression: Methods and Protocols. Humana Press. 2013. p. 337-350. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-526-2_22

Author

Hernandez-Verdun, Danièle ; Louvet, Emilie ; Muro, Eleonora. / Time-lapse, photoactivation, and photobleaching imaging of nucleolar assembly after mitosis. Imaging Gene Expression: Methods and Protocols. editor / Yaron Shav-Tal. Humana Press, 2013. pp. 337-350 (Methods in Molecular Biology).

Bibtex Download

@inbook{40a669ed51cb4d9dbcbd1af9127bc036,
title = "Time-lapse, photoactivation, and photobleaching imaging of nucleolar assembly after mitosis",
abstract = "Nucleolus assembly starts in telophase with the benefit of building blocks passing through mitosis and lasts until cytokinesis generating the two independent interphasic cells. Several approaches make it possible to follow the dynamics of fluorescent molecules in live cells. Here, three complementary approaches are described to measure the dynamics of proteins during nucleolar assembly after mitosis: (1) rapid two-color 4-D imaging time-lapse microscopy that demonstrates the relative localization and movement of two proteins, (2) photoactivation that reveals the directionality of migration from the activated area, and (3) fluorescence recovery after photobleaching (FRAP) that measures the renewing of proteins in the bleached area. We demonstrate that the order of recruitment of the processing machineries into nucleoli results from differential sorting of intermediate structures assembled during telophase, the prenucleolar bodies.",
keywords = "Cell cycle, Dynamics, FRAP, Live cell imaging, Nucleoli, Photoactivation, PNB",
author = "Dani{\`e}le Hernandez-Verdun and Emilie Louvet and Eleonora Muro",
year = "2013",
month = sep,
doi = "10.1007/978-1-62703-526-2_22",
language = "English",
isbn = "9781627035255",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "337--350",
editor = "Yaron Shav-Tal",
booktitle = "Imaging Gene Expression",

}

RIS (suitable for import to EndNote) Download

TY - CHAP

T1 - Time-lapse, photoactivation, and photobleaching imaging of nucleolar assembly after mitosis

AU - Hernandez-Verdun, Danièle

AU - Louvet, Emilie

AU - Muro, Eleonora

PY - 2013/9

Y1 - 2013/9

N2 - Nucleolus assembly starts in telophase with the benefit of building blocks passing through mitosis and lasts until cytokinesis generating the two independent interphasic cells. Several approaches make it possible to follow the dynamics of fluorescent molecules in live cells. Here, three complementary approaches are described to measure the dynamics of proteins during nucleolar assembly after mitosis: (1) rapid two-color 4-D imaging time-lapse microscopy that demonstrates the relative localization and movement of two proteins, (2) photoactivation that reveals the directionality of migration from the activated area, and (3) fluorescence recovery after photobleaching (FRAP) that measures the renewing of proteins in the bleached area. We demonstrate that the order of recruitment of the processing machineries into nucleoli results from differential sorting of intermediate structures assembled during telophase, the prenucleolar bodies.

AB - Nucleolus assembly starts in telophase with the benefit of building blocks passing through mitosis and lasts until cytokinesis generating the two independent interphasic cells. Several approaches make it possible to follow the dynamics of fluorescent molecules in live cells. Here, three complementary approaches are described to measure the dynamics of proteins during nucleolar assembly after mitosis: (1) rapid two-color 4-D imaging time-lapse microscopy that demonstrates the relative localization and movement of two proteins, (2) photoactivation that reveals the directionality of migration from the activated area, and (3) fluorescence recovery after photobleaching (FRAP) that measures the renewing of proteins in the bleached area. We demonstrate that the order of recruitment of the processing machineries into nucleoli results from differential sorting of intermediate structures assembled during telophase, the prenucleolar bodies.

KW - Cell cycle

KW - Dynamics

KW - FRAP

KW - Live cell imaging

KW - Nucleoli

KW - Photoactivation

KW - PNB

UR - http://www.scopus.com/inward/record.url?scp=84884145280&partnerID=8YFLogxK

U2 - 10.1007/978-1-62703-526-2_22

DO - 10.1007/978-1-62703-526-2_22

M3 - Chapter

C2 - 23980017

SN - 9781627035255

T3 - Methods in Molecular Biology

SP - 337

EP - 350

BT - Imaging Gene Expression

A2 - Shav-Tal, Yaron

PB - Humana Press

ER -

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