Tissue Specific Deletion of Inhibitor of Kappa B Kinase 2 with OX40-Cre Reveals the Unanticipated Expression from the OX40 Locus in Skin Epidermis

Georgina H. Cornish*, Sim L. Tung, Daniel Marshall, Steve Ley, Benedict P. Seddon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

NF-kappa B signalling plays an essential role in T cell activation and generation of regulatory and memory populations in vivo. In the present study, we aimed to investigate the role of NF-kappa B signalling in post-activation T cells using tissue specific ablation of inhibitor of kappa-B kinase 2 expression, an important component of the inhibitor of kappa-B kinase complex in canonical NF-kappa B signalling. The OX40 antigen is expressed on activated T cells. Therefore, we used previously described mouse strain expressing Cre recombinase from the endogenous OX40 locus. Ablation of IKK2 expression using OX40(Cre) mice resulted in the development of an inflammatory response in the skin epidermis causing wide spread skin lesions. The inflammatory response was characterised by extensive leukocytic infiltrate in skin tissue, hyperplasia of draining lymph nodes and widespread activation in the T cell compartment. Surprisingly, disease development did not depend on T cells but was rather associated with an unanticipated expression of Cre in skin epidermis, and activation of the T cell compartment did not require Ikbk2 deletion in T cells. Employment of Cre reporter strains revealed extensive Cre activity in skin epidermis. Therefore, development of skin lesions was rather more likely explained by deletion of Ikbk2 in skin keratinocytes in OX40(Cre) mice.

Original languageEnglish
Article numbere32193
Number of pages10
JournalPL o S One
Volume7
Issue number2
DOIs
Publication statusPublished - 21 Feb 2012

Keywords

  • T-CELLS
  • IKK COMPLEX
  • MOLECULAR CHARACTERIZATION
  • INCONTINENTIA PIGMENTI
  • LIVER DEGENERATION
  • MICE LACKING
  • IN-VIVO
  • ACTIVATION
  • INFLAMMATION
  • COMPONENT

Fingerprint

Dive into the research topics of 'Tissue Specific Deletion of Inhibitor of Kappa B Kinase 2 with OX40-Cre Reveals the Unanticipated Expression from the OX40 Locus in Skin Epidermis'. Together they form a unique fingerprint.

Cite this