Towards high-throughput FLIM for protein-protein interaction screening of live cells and tissue microarrays

P R Barber; G P Pierce; Simon Ameer-Beg; Daniel Matthews; Leo Carlin; Melanie Keppler; Frederic Festy; C. Gillett; R Springall; Tony Ng; Boris Vojnovic

doi:10.1109/ISBI.2008.4541006

Towards high-throughput FLIM for protein-protein interaction screening of live cells and tissue microarrays

P R Barber, G P Pierce, Simon Ameer-Beg, Daniel Matthews, Leo Carlin, Melanie Keppler, Frederic Festy, C. Gillett, R Springall, Tony Ng, Boris Vojnovic

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Research output: Chapter in Book/Report/Conference proceeding › Conference paper

4 Citations (Scopus)

Abstract

Studying cellular protein-protein interactions in situ requires a technique such as fluorescence resonance energy transfer (FRET) which is sensitive on the nanometer scale. Observing FRET is significantly simplified if the fluorescence lifetime of the donor can be monitored. Results from live cells and tissue micro arrays are presented from an automated microscope incorporating time-domain TCSPC fluorescence lifetime imaging (FLIM). Novel hardware and software with a modular approach and scripting abilities allow us to work towards speed-optimized acquisition and ease of use to bring FLIM into the high-throughput regime.

Original language	English
Title of host publication	Biomedical Imaging: From Nano to Macro
Publisher	IEEE
Pages	356-359
Number of pages	4
ISBN (Electronic)	978-1-4244-2003-2
ISBN (Print)	978-1-4244-2002-5
DOIs	https://doi.org/10.1109/ISBI.2008.4541006
Publication status	Published - 2008

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10.1109/ISBI.2008.4541006

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title = "Towards high-throughput FLIM for protein-protein interaction screening of live cells and tissue microarrays",

abstract = "Studying cellular protein-protein interactions in situ requires a technique such as fluorescence resonance energy transfer (FRET) which is sensitive on the nanometer scale. Observing FRET is significantly simplified if the fluorescence lifetime of the donor can be monitored. Results from live cells and tissue micro arrays are presented from an automated microscope incorporating time-domain TCSPC fluorescence lifetime imaging (FLIM). Novel hardware and software with a modular approach and scripting abilities allow us to work towards speed-optimized acquisition and ease of use to bring FLIM into the high-throughput regime.",

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doi = "10.1109/ISBI.2008.4541006",

language = "English",

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T1 - Towards high-throughput FLIM for protein-protein interaction screening of live cells and tissue microarrays

AU - Barber, P R

AU - Pierce, G P

AU - Ameer-Beg, Simon

AU - Matthews, Daniel

AU - Carlin, Leo

AU - Keppler, Melanie

AU - Festy, Frederic

AU - Gillett, C.

AU - Springall, R

AU - Ng, Tony

AU - Vojnovic, Boris

PY - 2008

Y1 - 2008

N2 - Studying cellular protein-protein interactions in situ requires a technique such as fluorescence resonance energy transfer (FRET) which is sensitive on the nanometer scale. Observing FRET is significantly simplified if the fluorescence lifetime of the donor can be monitored. Results from live cells and tissue micro arrays are presented from an automated microscope incorporating time-domain TCSPC fluorescence lifetime imaging (FLIM). Novel hardware and software with a modular approach and scripting abilities allow us to work towards speed-optimized acquisition and ease of use to bring FLIM into the high-throughput regime.

AB - Studying cellular protein-protein interactions in situ requires a technique such as fluorescence resonance energy transfer (FRET) which is sensitive on the nanometer scale. Observing FRET is significantly simplified if the fluorescence lifetime of the donor can be monitored. Results from live cells and tissue micro arrays are presented from an automated microscope incorporating time-domain TCSPC fluorescence lifetime imaging (FLIM). Novel hardware and software with a modular approach and scripting abilities allow us to work towards speed-optimized acquisition and ease of use to bring FLIM into the high-throughput regime.

U2 - 10.1109/ISBI.2008.4541006

DO - 10.1109/ISBI.2008.4541006

M3 - Conference paper

SN - 978-1-4244-2002-5

SP - 356

EP - 359

BT - Biomedical Imaging: From Nano to Macro

PB - IEEE

ER -

Towards high-throughput FLIM for protein-protein interaction screening of live cells and tissue microarrays

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