Toxicogenomics of iron oxide nanoparticles in the nematode C. elegans

Laura Gonzalez-Moragas; Si-Ming Yu; Núria Benseny-Cases; Stephen Stürzenbaum; Anna Roig; Anna Laromaine

doi:10.1080/17435390.2017.1342011

Toxicogenomics of iron oxide nanoparticles in the nematode C. elegans

Laura Gonzalez-Moragas, Si-Ming Yu, Núria Benseny-Cases, Stephen Stürzenbaum, Anna Roig, Anna Laromaine

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

618 Downloads (Pure)

Abstract

We present a mechanistic study of the effect of iron oxide nanoparticles (SPIONs) in Caenorhabditis elegans combining a genome-wide analysis with the investigation of specific molecular markers frequently linked to nanotoxicity. The effects of two different coatings were explored: citrate, an anionic stabilizer, and bovine serum albumin, as a pre-formed protein corona. The transcriptomic study identified differentially expressed genes following an exposure to SPIONs. The expression of genes involved in oxidative stress, metal detoxification response, endocytosis, intestinal integrity and iron homeostasis was quantitatively evaluated. The role of oxidative stress was confirmed by gene expression analysis and by synchrotron Fourier Transform infrared microscopy based on the higher tissue oxidation of NP-treated animals. The observed transcriptional modulation of key signaling pathways such as MAPK and Wnt suggests that SPIONs might be endocytosed by clathrin-mediated processes, a putative mechanism of nanotoxicity which deserves further mechanistic investigations.

Original language	English
Pages (from-to)	1-11
Number of pages	11
Journal	Nanotoxicology
Early online date	4 Jul 2017
DOIs	https://doi.org/10.1080/17435390.2017.1342011
Publication status	E-pub ahead of print - 4 Jul 2017

Keywords

Journal Article

Access to Document

10.1080/17435390.2017.1342011

Toxicogenomics of iron oxide_GONZALEZ-MORAGAS_Publishedonline4July2017_GREEN AAMAccepted author manuscript, 2.52 MB

Cite this

@article{00c3e8b3304745929df77c5f1da26e3d,

title = "Toxicogenomics of iron oxide nanoparticles in the nematode C. elegans",

abstract = "We present a mechanistic study of the effect of iron oxide nanoparticles (SPIONs) in Caenorhabditis elegans combining a genome-wide analysis with the investigation of specific molecular markers frequently linked to nanotoxicity. The effects of two different coatings were explored: citrate, an anionic stabilizer, and bovine serum albumin, as a pre-formed protein corona. The transcriptomic study identified differentially expressed genes following an exposure to SPIONs. The expression of genes involved in oxidative stress, metal detoxification response, endocytosis, intestinal integrity and iron homeostasis was quantitatively evaluated. The role of oxidative stress was confirmed by gene expression analysis and by synchrotron Fourier Transform infrared microscopy based on the higher tissue oxidation of NP-treated animals. The observed transcriptional modulation of key signaling pathways such as MAPK and Wnt suggests that SPIONs might be endocytosed by clathrin-mediated processes, a putative mechanism of nanotoxicity which deserves further mechanistic investigations.",

keywords = "Journal Article",

author = "Laura Gonzalez-Moragas and Si-Ming Yu and N{\'u}ria Benseny-Cases and Stephen St{\"u}rzenbaum and Anna Roig and Anna Laromaine",

year = "2017",

month = jul,

day = "4",

doi = "10.1080/17435390.2017.1342011",

language = "English",

pages = "1--11",

journal = "Nanotoxicology",

issn = "1743-5390",

publisher = "Informa Healthcare",

}

TY - JOUR

T1 - Toxicogenomics of iron oxide nanoparticles in the nematode C. elegans

AU - Gonzalez-Moragas, Laura

AU - Yu, Si-Ming

AU - Benseny-Cases, Núria

AU - Stürzenbaum, Stephen

AU - Roig, Anna

AU - Laromaine, Anna

PY - 2017/7/4

Y1 - 2017/7/4

N2 - We present a mechanistic study of the effect of iron oxide nanoparticles (SPIONs) in Caenorhabditis elegans combining a genome-wide analysis with the investigation of specific molecular markers frequently linked to nanotoxicity. The effects of two different coatings were explored: citrate, an anionic stabilizer, and bovine serum albumin, as a pre-formed protein corona. The transcriptomic study identified differentially expressed genes following an exposure to SPIONs. The expression of genes involved in oxidative stress, metal detoxification response, endocytosis, intestinal integrity and iron homeostasis was quantitatively evaluated. The role of oxidative stress was confirmed by gene expression analysis and by synchrotron Fourier Transform infrared microscopy based on the higher tissue oxidation of NP-treated animals. The observed transcriptional modulation of key signaling pathways such as MAPK and Wnt suggests that SPIONs might be endocytosed by clathrin-mediated processes, a putative mechanism of nanotoxicity which deserves further mechanistic investigations.

AB - We present a mechanistic study of the effect of iron oxide nanoparticles (SPIONs) in Caenorhabditis elegans combining a genome-wide analysis with the investigation of specific molecular markers frequently linked to nanotoxicity. The effects of two different coatings were explored: citrate, an anionic stabilizer, and bovine serum albumin, as a pre-formed protein corona. The transcriptomic study identified differentially expressed genes following an exposure to SPIONs. The expression of genes involved in oxidative stress, metal detoxification response, endocytosis, intestinal integrity and iron homeostasis was quantitatively evaluated. The role of oxidative stress was confirmed by gene expression analysis and by synchrotron Fourier Transform infrared microscopy based on the higher tissue oxidation of NP-treated animals. The observed transcriptional modulation of key signaling pathways such as MAPK and Wnt suggests that SPIONs might be endocytosed by clathrin-mediated processes, a putative mechanism of nanotoxicity which deserves further mechanistic investigations.

KW - Journal Article

U2 - 10.1080/17435390.2017.1342011

DO - 10.1080/17435390.2017.1342011

M3 - Article

C2 - 28673184

SN - 1743-5390

SP - 1

EP - 11

JO - Nanotoxicology

JF - Nanotoxicology

ER -

Toxicogenomics of iron oxide nanoparticles in the nematode C. elegans

Abstract

Keywords

Access to Document

Fingerprint

Cite this