Tracking displacements of intracellular organelles in response to nanomechanical forces

TY - CHAP

T1 - Tracking displacements of intracellular organelles in response to nanomechanical forces

AU - Silberberg, Yaron R.

AU - Pelling, Andrew E.

AU - Yakubov, Gleb E.

AU - Crum, William

AU - Hawkes, David J.

AU - Horton, Mike A.

PY - 2008

Y1 - 2008

N2 - The living cell is under constant influence of mechanical forces from its environment. These forces affect many aspects of the cell's behaviour, including morphology, growth, cell differentiation, protein synthesis and cell death. In this study we show how mechanical stress perturbs the intracellular structures of the cell and induces mechanical responses. In order to correlate mechanical perturbations to cellular responses, we used a combined fluorescence-atomic force microscope (AFM) to produce nanomechanical perturbations while simultaneously tracking the real-time motion of fluorescently labelled mitochondria in live cells. Feature point tracking was then used to analyze and quantify the structural displacements. Following indentation from the AFM tip, the average mitochondrial displacement showed an increase of similar to 40% in comparison to the natural movement. These results show how mitochondrial structures that are far away from the point of force (up to similar to 40 mu m) are instantaneously affected by extracellular perturbations.

AB - The living cell is under constant influence of mechanical forces from its environment. These forces affect many aspects of the cell's behaviour, including morphology, growth, cell differentiation, protein synthesis and cell death. In this study we show how mechanical stress perturbs the intracellular structures of the cell and induces mechanical responses. In order to correlate mechanical perturbations to cellular responses, we used a combined fluorescence-atomic force microscope (AFM) to produce nanomechanical perturbations while simultaneously tracking the real-time motion of fluorescently labelled mitochondria in live cells. Feature point tracking was then used to analyze and quantify the structural displacements. Following indentation from the AFM tip, the average mitochondrial displacement showed an increase of similar to 40% in comparison to the natural movement. These results show how mitochondrial structures that are far away from the point of force (up to similar to 40 mu m) are instantaneously affected by extracellular perturbations.

U2 - 10.1109/ISBI.2008.4541251

DO - 10.1109/ISBI.2008.4541251

M3 - Conference paper

SN - 978-1-4244-2002-5

SP - 1335

EP - 1338

BT - 2008 IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING: FROM NANO TO MACRO, VOLS 1-4

PB - IEEE

CY - NEW YORK

T2 - 5th IEEE International Symposium on Biomedical Imaging - From Nano to Macro

Y2 - 14 May 2008 through 17 May 2008

ER -