Tracking displacements of intracellular organelles in response to nanomechanical forces

Yaron R. Silberberg, Andrew E. Pelling, Gleb E. Yakubov, William Crum, David J. Hawkes, Mike A. Horton

Research output: Chapter in Book/Report/Conference proceedingConference paper

5 Citations (Scopus)

Abstract

The living cell is under constant influence of mechanical forces from its environment. These forces affect many aspects of the cell's behaviour, including morphology, growth, cell differentiation, protein synthesis and cell death. In this study we show how mechanical stress perturbs the intracellular structures of the cell and induces mechanical responses. In order to correlate mechanical perturbations to cellular responses, we used a combined fluorescence-atomic force microscope (AFM) to produce nanomechanical perturbations while simultaneously tracking the real-time motion of fluorescently labelled mitochondria in live cells. Feature point tracking was then used to analyze and quantify the structural displacements. Following indentation from the AFM tip, the average mitochondrial displacement showed an increase of similar to 40% in comparison to the natural movement. These results show how mitochondrial structures that are far away from the point of force (up to similar to 40 mu m) are instantaneously affected by extracellular perturbations.

Original languageEnglish
Title of host publication2008 IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING: FROM NANO TO MACRO, VOLS 1-4
Place of PublicationNEW YORK
PublisherIEEE
Pages1335-1338
Number of pages4
ISBN (Print)978-1-4244-2002-5
DOIs
Publication statusPublished - 2008
Event5th IEEE International Symposium on Biomedical Imaging - From Nano to Macro - Paris
Duration: 14 May 200817 May 2008

Conference

Conference5th IEEE International Symposium on Biomedical Imaging - From Nano to Macro
CityParis
Period14/05/200817/05/2008

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