Tracking glycosylation in live cells using FTIR spectroscopy

Joshua  Phelan; Ali Ibrahim Altharawi; Andrew Chan

doi:10.1016/j.talanta.2020.120737

Tracking glycosylation in live cells using FTIR spectroscopy

Joshua Phelan, Ali Ibrahim Altharawi, Andrew Chan

Institute of Pharmaceutical Science

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Abstract

This is the first demonstration of the study of glycan protein turnover in living cells by FTIR with commercially available tetraacetylated N-Azidoacetyl-D-Mannosamine (Ac4ManNAz) label. The FTIR analysis has shown to be able to monitor the metabolism of glycans in living cells in real time. The method is simple, quantitative and requires equipment that are available in many laboratories. It can be used in a wide range of applications such as the study of glycosylation and cell-signalling.

Original language	English
Article number	120737
Journal	TALANTA
Volume	211
Early online date	13 Jan 2020
DOIs	https://doi.org/10.1016/j.talanta.2020.120737
Publication status	Published - May 2020

Keywords

ATR
Alkyne
Azide
FT-IR
Live cells
Metabolic labelling
Vibrational spectroscopy

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10.1016/j.talanta.2020.120737

Tracking glycosylation in live_PHELAN_Acc11Jan2020Epub13Jan2020_GREEN AAMAccepted author manuscript, 458 KBLicence: CC BY-NC-ND

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abstract = "This is the first demonstration of the study of glycan protein turnover in living cells by FTIR with commercially available tetraacetylated N-Azidoacetyl-D-Mannosamine (Ac4ManNAz) label. The FTIR analysis has shown to be able to monitor the metabolism of glycans in living cells in real time. The method is simple, quantitative and requires equipment that are available in many laboratories. It can be used in a wide range of applications such as the study of glycosylation and cell-signalling. ",

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AU - Phelan, Joshua

AU - Altharawi, Ali Ibrahim

AU - Chan, Andrew

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AB - This is the first demonstration of the study of glycan protein turnover in living cells by FTIR with commercially available tetraacetylated N-Azidoacetyl-D-Mannosamine (Ac4ManNAz) label. The FTIR analysis has shown to be able to monitor the metabolism of glycans in living cells in real time. The method is simple, quantitative and requires equipment that are available in many laboratories. It can be used in a wide range of applications such as the study of glycosylation and cell-signalling.

KW - ATR

KW - Alkyne

KW - Azide

KW - FT-IR

KW - Live cells

KW - Metabolic labelling

KW - Vibrational spectroscopy

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VL - 211

JO - TALANTA

JF - TALANTA

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ER -

Tracking glycosylation in live cells using FTIR spectroscopy

Abstract

Keywords

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