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Transcriptome profiling of the fungus Aspergillus nidulans exposed to a commercial glyphosate-based herbicide under conditions of apparent herbicide tolerance

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Transcriptome profiling of the fungus Aspergillus nidulans exposed to a commercial glyphosate-based herbicide under conditions of apparent herbicide tolerance. / Mesnage, Robin; Oestreicher, Nathalie; Poirier, Florence; Nicolas, Valérie; Boursier, Céline; Vélot, Christian.

In: Environmental Research, Vol. 182, 109116, 03.2020.

Research output: Contribution to journalArticlepeer-review

Harvard

Mesnage, R, Oestreicher, N, Poirier, F, Nicolas, V, Boursier, C & Vélot, C 2020, 'Transcriptome profiling of the fungus Aspergillus nidulans exposed to a commercial glyphosate-based herbicide under conditions of apparent herbicide tolerance', Environmental Research, vol. 182, 109116. https://doi.org/10.1016/j.envres.2020.109116

APA

Mesnage, R., Oestreicher, N., Poirier, F., Nicolas, V., Boursier, C., & Vélot, C. (2020). Transcriptome profiling of the fungus Aspergillus nidulans exposed to a commercial glyphosate-based herbicide under conditions of apparent herbicide tolerance. Environmental Research, 182, [109116]. https://doi.org/10.1016/j.envres.2020.109116

Vancouver

Mesnage R, Oestreicher N, Poirier F, Nicolas V, Boursier C, Vélot C. Transcriptome profiling of the fungus Aspergillus nidulans exposed to a commercial glyphosate-based herbicide under conditions of apparent herbicide tolerance. Environmental Research. 2020 Mar;182. 109116. https://doi.org/10.1016/j.envres.2020.109116

Author

Mesnage, Robin ; Oestreicher, Nathalie ; Poirier, Florence ; Nicolas, Valérie ; Boursier, Céline ; Vélot, Christian. / Transcriptome profiling of the fungus Aspergillus nidulans exposed to a commercial glyphosate-based herbicide under conditions of apparent herbicide tolerance. In: Environmental Research. 2020 ; Vol. 182.

Bibtex Download

@article{bd15900f02bb42d19932dea014263a19,
title = "Transcriptome profiling of the fungus Aspergillus nidulans exposed to a commercial glyphosate-based herbicide under conditions of apparent herbicide tolerance",
abstract = "Glyphosate-based herbicides, such as Roundup{\textregistered}, are the most widely used non-selective, broad-spectrum herbicides. The release of these compounds in large amounts into the environment is susceptible to affect soil quality and health, especially because of the non-target effects on a large range of organisms including soil microorganisms. The soil filamentous fungus Aspergillus nidulans, a well-characterized experimental model organism that can be used as a bio-indicator for agricultural soil health, has been previously shown to be highly affected by Roundup GT Plus (R450: 450 g/L of glyphosate) at concentrations far below recommended agricultural application rate, including at a dose that does not cause any macroscopic effect. In this study, we determined alterations in the transcriptome of A. nidulans when exposed to R450 at a dose corresponding to the no-observed-adverse-effect level (NOAEL) for macroscopic parameters. A total of 1816 distinct genes had their expression altered. The most affected biological functions were protein synthesis, amino acids and secondary metabolisms, stress response, as well as detoxification pathways through cytochromes P450, glutathione-S-transferases, and ABC transporters. These results partly explain the molecular mechanisms underlying alterations in growth parameters detected at higher concentrations for this ascomycete fungus. In conclusion, our results highlight molecular disturbances in a soil fungus under conditions of apparent tolerance to the herbicide, and thus confirm the need to question the principle of “substantial equivalence” when applied to plants made tolerant to herbicides.",
keywords = "Apparent herbicide tolerance, Aspergillus nidulans, Glyphosate-based herbicide, Substantial equivalence, Transcriptomics",
author = "Robin Mesnage and Nathalie Oestreicher and Florence Poirier and Val{\'e}rie Nicolas and C{\'e}line Boursier and Christian V{\'e}lot",
year = "2020",
month = mar,
doi = "10.1016/j.envres.2020.109116",
language = "English",
volume = "182",
journal = "Environmental Research",
issn = "0013-9351",
publisher = "ACADEMIC PRESS INC",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Transcriptome profiling of the fungus Aspergillus nidulans exposed to a commercial glyphosate-based herbicide under conditions of apparent herbicide tolerance

AU - Mesnage, Robin

AU - Oestreicher, Nathalie

AU - Poirier, Florence

AU - Nicolas, Valérie

AU - Boursier, Céline

AU - Vélot, Christian

PY - 2020/3

Y1 - 2020/3

N2 - Glyphosate-based herbicides, such as Roundup®, are the most widely used non-selective, broad-spectrum herbicides. The release of these compounds in large amounts into the environment is susceptible to affect soil quality and health, especially because of the non-target effects on a large range of organisms including soil microorganisms. The soil filamentous fungus Aspergillus nidulans, a well-characterized experimental model organism that can be used as a bio-indicator for agricultural soil health, has been previously shown to be highly affected by Roundup GT Plus (R450: 450 g/L of glyphosate) at concentrations far below recommended agricultural application rate, including at a dose that does not cause any macroscopic effect. In this study, we determined alterations in the transcriptome of A. nidulans when exposed to R450 at a dose corresponding to the no-observed-adverse-effect level (NOAEL) for macroscopic parameters. A total of 1816 distinct genes had their expression altered. The most affected biological functions were protein synthesis, amino acids and secondary metabolisms, stress response, as well as detoxification pathways through cytochromes P450, glutathione-S-transferases, and ABC transporters. These results partly explain the molecular mechanisms underlying alterations in growth parameters detected at higher concentrations for this ascomycete fungus. In conclusion, our results highlight molecular disturbances in a soil fungus under conditions of apparent tolerance to the herbicide, and thus confirm the need to question the principle of “substantial equivalence” when applied to plants made tolerant to herbicides.

AB - Glyphosate-based herbicides, such as Roundup®, are the most widely used non-selective, broad-spectrum herbicides. The release of these compounds in large amounts into the environment is susceptible to affect soil quality and health, especially because of the non-target effects on a large range of organisms including soil microorganisms. The soil filamentous fungus Aspergillus nidulans, a well-characterized experimental model organism that can be used as a bio-indicator for agricultural soil health, has been previously shown to be highly affected by Roundup GT Plus (R450: 450 g/L of glyphosate) at concentrations far below recommended agricultural application rate, including at a dose that does not cause any macroscopic effect. In this study, we determined alterations in the transcriptome of A. nidulans when exposed to R450 at a dose corresponding to the no-observed-adverse-effect level (NOAEL) for macroscopic parameters. A total of 1816 distinct genes had their expression altered. The most affected biological functions were protein synthesis, amino acids and secondary metabolisms, stress response, as well as detoxification pathways through cytochromes P450, glutathione-S-transferases, and ABC transporters. These results partly explain the molecular mechanisms underlying alterations in growth parameters detected at higher concentrations for this ascomycete fungus. In conclusion, our results highlight molecular disturbances in a soil fungus under conditions of apparent tolerance to the herbicide, and thus confirm the need to question the principle of “substantial equivalence” when applied to plants made tolerant to herbicides.

KW - Apparent herbicide tolerance

KW - Aspergillus nidulans

KW - Glyphosate-based herbicide

KW - Substantial equivalence

KW - Transcriptomics

UR - http://www.scopus.com/inward/record.url?scp=85077922021&partnerID=8YFLogxK

U2 - 10.1016/j.envres.2020.109116

DO - 10.1016/j.envres.2020.109116

M3 - Article

AN - SCOPUS:85077922021

VL - 182

JO - Environmental Research

JF - Environmental Research

SN - 0013-9351

M1 - 109116

ER -

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