Research output: Contribution to journal › Article › peer-review
Artur Bryja, Grzegorz Latosiński, Maurycy Jankowski, Ana Angelova Volponi, Paul Mozdziak, Jamil A. Shibli, Rut Bryl, Julia Spaczyńska, Hanna Piotrowska-Kempisty, Krzysztof Krawiec, Bartosz Kempisty, Marta Dyszkiewicz-Konwińska
Original language | English |
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Article number | 15 |
Pages (from-to) | 1-23 |
Number of pages | 23 |
Journal | Animals |
Volume | 11 |
Issue number | 1 |
DOIs | |
Published | Jan 2021 |
Additional links |
Transcriptional analysis and live-cell imaging are a powerful tool to investigate the dynamics of complex biological systems. In vitro expanded porcine oral mucosal cells, consisting of populations of epithelial and connective lineages, are interesting and complex systems for study via microarray transcriptomic assays to analyze gene expression profile. The transcriptomic analysis included 56 ontological groups with particular focus on 7 gene ontology groups that are related to the processes of differentiation and development. Most analyzed genes were upregulated after 7 days and downregulated after 15 and 30 days of in vitro culture. The performed transcriptomic analysis was then extended to include automated analysis of differential interference contrast microscopy (DIC) images obtained during in vitro culture. The analysis of DIC imaging allowed to identify the different populations of keratinocytes and fibroblasts during seven days of in vitro culture, and it was possible to evaluate the proportion of these two populations of cells. Porcine mucosa may be a suitable model for reference research on human tissues. In addition, it can provide a reference point for research on the use of cells, scaffolds, or tissues derived from transgenic animals for applications in human tissues reconstruction.
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