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Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration

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Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration. / Hong, Xuechong; Margariti, Andriana; Le Bras, Alexandra; Jacquet, Laureen; Kong, Wei; Hu, Yanhua; Xu, Qingbo.

In: Scientific Reports, Vol. 7, No. 1, 5590, 17.07.2017.

Research output: Contribution to journalArticle

Harvard

Hong, X, Margariti, A, Le Bras, A, Jacquet, L, Kong, W, Hu, Y & Xu, Q 2017, 'Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration', Scientific Reports, vol. 7, no. 1, 5590. https://doi.org/10.1038/s41598-017-05665-7

APA

Hong, X., Margariti, A., Le Bras, A., Jacquet, L., Kong, W., Hu, Y., & Xu, Q. (2017). Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration. Scientific Reports, 7(1), [5590]. https://doi.org/10.1038/s41598-017-05665-7

Vancouver

Hong X, Margariti A, Le Bras A, Jacquet L, Kong W, Hu Y et al. Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration. Scientific Reports. 2017 Jul 17;7(1). 5590. https://doi.org/10.1038/s41598-017-05665-7

Author

Hong, Xuechong ; Margariti, Andriana ; Le Bras, Alexandra ; Jacquet, Laureen ; Kong, Wei ; Hu, Yanhua ; Xu, Qingbo. / Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration. In: Scientific Reports. 2017 ; Vol. 7, No. 1.

Bibtex Download

@article{5a4dfa92e8064f849a27567e8965a886,
title = "Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration",
abstract = "Endothelial dysfunction is widely implicated in cardiovascular pathological changes and development of vascular disease. In view of the fact that the spontaneous endothelial cell (EC) regeneration is a slow and insufficient process, it is of great interest to explore alternative cell sources capable of generating functional ECs. Vascular smooth muscle cell (SMC) composes the majority of the vascular wall and retains phenotypic plasticity in response to various stimuli. The aim of this study is to test the feasibility of the conversion of SMC into functional EC through the use of reprogramming factors. Human SMCs are first dedifferentiated for 4 days to achieve a vascular progenitor state expressing CD34, by introducing transcription factors OCT4, SOX2, KLF4 and c-MYC. These SMC-derived progenitors are then differentiated along the endothelial lineage. The SMC-converted ECs exhibit typical endothelial markers expression and endothelial functions in vitro, in vivo and in disease model. Further comprehensive analysis indicates that mesenchymal-to-epithelial transition is requisite to initiate SMCs reprogramming into vascular progenitors and that members of the Notch signalling pathway regulate further differentiation of the progenitors into endothelial lineage. Together, we provide the first evidence of the feasibility of the conversion of human SMCs towards endothelial lineage through an intermediate vascular progenitor state induced by reprogramming.",
author = "Xuechong Hong and Andriana Margariti and {Le Bras}, Alexandra and Laureen Jacquet and Wei Kong and Yanhua Hu and Qingbo Xu",
year = "2017",
month = "7",
day = "17",
doi = "10.1038/s41598-017-05665-7",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS (suitable for import to EndNote) Download

TY - JOUR

T1 - Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration

AU - Hong, Xuechong

AU - Margariti, Andriana

AU - Le Bras, Alexandra

AU - Jacquet, Laureen

AU - Kong, Wei

AU - Hu, Yanhua

AU - Xu, Qingbo

PY - 2017/7/17

Y1 - 2017/7/17

N2 - Endothelial dysfunction is widely implicated in cardiovascular pathological changes and development of vascular disease. In view of the fact that the spontaneous endothelial cell (EC) regeneration is a slow and insufficient process, it is of great interest to explore alternative cell sources capable of generating functional ECs. Vascular smooth muscle cell (SMC) composes the majority of the vascular wall and retains phenotypic plasticity in response to various stimuli. The aim of this study is to test the feasibility of the conversion of SMC into functional EC through the use of reprogramming factors. Human SMCs are first dedifferentiated for 4 days to achieve a vascular progenitor state expressing CD34, by introducing transcription factors OCT4, SOX2, KLF4 and c-MYC. These SMC-derived progenitors are then differentiated along the endothelial lineage. The SMC-converted ECs exhibit typical endothelial markers expression and endothelial functions in vitro, in vivo and in disease model. Further comprehensive analysis indicates that mesenchymal-to-epithelial transition is requisite to initiate SMCs reprogramming into vascular progenitors and that members of the Notch signalling pathway regulate further differentiation of the progenitors into endothelial lineage. Together, we provide the first evidence of the feasibility of the conversion of human SMCs towards endothelial lineage through an intermediate vascular progenitor state induced by reprogramming.

AB - Endothelial dysfunction is widely implicated in cardiovascular pathological changes and development of vascular disease. In view of the fact that the spontaneous endothelial cell (EC) regeneration is a slow and insufficient process, it is of great interest to explore alternative cell sources capable of generating functional ECs. Vascular smooth muscle cell (SMC) composes the majority of the vascular wall and retains phenotypic plasticity in response to various stimuli. The aim of this study is to test the feasibility of the conversion of SMC into functional EC through the use of reprogramming factors. Human SMCs are first dedifferentiated for 4 days to achieve a vascular progenitor state expressing CD34, by introducing transcription factors OCT4, SOX2, KLF4 and c-MYC. These SMC-derived progenitors are then differentiated along the endothelial lineage. The SMC-converted ECs exhibit typical endothelial markers expression and endothelial functions in vitro, in vivo and in disease model. Further comprehensive analysis indicates that mesenchymal-to-epithelial transition is requisite to initiate SMCs reprogramming into vascular progenitors and that members of the Notch signalling pathway regulate further differentiation of the progenitors into endothelial lineage. Together, we provide the first evidence of the feasibility of the conversion of human SMCs towards endothelial lineage through an intermediate vascular progenitor state induced by reprogramming.

UR - http://www.scopus.com/inward/record.url?scp=85025135872&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-05665-7

DO - 10.1038/s41598-017-05665-7

M3 - Article

AN - SCOPUS:85025135872

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 5590

ER -

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