Transient changes in cortical glucose and lactate levels associated with peri-infarct depolarisations, studied with rapid-sampling microdialysis

TY - JOUR

T1 - Transient changes in cortical glucose and lactate levels associated with peri-infarct depolarisations, studied with rapid-sampling microdialysis

AU - Hopwood, S E

AU - Parkin, M C

AU - Bezzina, E L

AU - Boutelle, M G

AU - Strong, A J

PY - 2005/3

Y1 - 2005/3

N2 - Peri-infarct depolarisations (PIDs) contribute to infarct expansion in experimental focal ischaemia; furthermore, depolarisations propagate in the injured human brain. Glucose utilisation is increased under both conditions, and depletion of brain glucose carries a poor prognosis. We studied dynamics of cerebral glucose and lactate in relation to PID patterns in experimental stroke. The middle cerebral artery was occluded for 3 h in 23 cats under terminal chloralose anaesthesia. We used fluorescence imaging to detect occurrence of PIDs, and rapid-sampling online microdialysis (rsMD), coupled to a flow-injection assay, to examine changes in cerebral cortical extracellular glucose and lactate at intervals of 30 sec each. After 30 min' ischaemia, lactate had increased by 43.6 +/- s.d. 45.9 mu mol/L, and stabilised in that range for 3 h. In contrast, glucose fell only slightly initially (11.9 +/- 9.7 mu mol/L), but progressively decreased to a reduction of 56.7 +/- 47.2 mu mol/L at 3 h, with no evidence of stabilisation. There was a highly significant inverse relationship of frequency of PIDs with plasma glucose (P

AB - Peri-infarct depolarisations (PIDs) contribute to infarct expansion in experimental focal ischaemia; furthermore, depolarisations propagate in the injured human brain. Glucose utilisation is increased under both conditions, and depletion of brain glucose carries a poor prognosis. We studied dynamics of cerebral glucose and lactate in relation to PID patterns in experimental stroke. The middle cerebral artery was occluded for 3 h in 23 cats under terminal chloralose anaesthesia. We used fluorescence imaging to detect occurrence of PIDs, and rapid-sampling online microdialysis (rsMD), coupled to a flow-injection assay, to examine changes in cerebral cortical extracellular glucose and lactate at intervals of 30 sec each. After 30 min' ischaemia, lactate had increased by 43.6 +/- s.d. 45.9 mu mol/L, and stabilised in that range for 3 h. In contrast, glucose fell only slightly initially (11.9 +/- 9.7 mu mol/L), but progressively decreased to a reduction of 56.7 +/- 47.2 mu mol/L at 3 h, with no evidence of stabilisation. There was a highly significant inverse relationship of frequency of PIDs with plasma glucose (P

U2 - 10.1038/sj.jcbfm.9600050

DO - 10.1038/sj.jcbfm.9600050

M3 - Article

SN - 1559-7016

VL - 25

SP - 391

EP - 401

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

IS - 3

ER -