TY - JOUR
T1 - Ubiquitous Chromatin-opening Elements (UCOEs)
T2 - Applications in biomanufacturing and gene therapy
AU - Neville, Jonathan J.
AU - Orlando, Joe
AU - Mann, Kimberly
AU - McCloskey, Bethany
AU - Antoniou, Michael N.
PY - 2017/9
Y1 - 2017/9
N2 - Ubiquitous Chromatin-opening Elements (UCOEs) are defined by their ability to consistently confer stable, site of integration-independent transgene expression that is proportional to copy number, including from within regions of heterochromatin such as centromeres. UCOEs structurally consist of methylation-free CpG islands encompassing single or dual divergently-transcribed housekeeping gene promoters. Since their discovery in 1999, UCOEs and their sub-fragments have found applications in areas of biotechnology requiring stable, reproducible, and high levels of gene expression. This review recounts the discovery of UCOEs and examines their current and future applications in protein therapeutic biomanufacturing and gene therapy.
AB - Ubiquitous Chromatin-opening Elements (UCOEs) are defined by their ability to consistently confer stable, site of integration-independent transgene expression that is proportional to copy number, including from within regions of heterochromatin such as centromeres. UCOEs structurally consist of methylation-free CpG islands encompassing single or dual divergently-transcribed housekeeping gene promoters. Since their discovery in 1999, UCOEs and their sub-fragments have found applications in areas of biotechnology requiring stable, reproducible, and high levels of gene expression. This review recounts the discovery of UCOEs and examines their current and future applications in protein therapeutic biomanufacturing and gene therapy.
KW - Ubiquitous chromatin opening element
KW - UCOE
KW - Biomanufacturing
KW - Gene therapy
KW - Stable gene expression
U2 - 10.1016/j.biotechadv.2017.05.004
DO - 10.1016/j.biotechadv.2017.05.004
M3 - Article
SN - 0734-9750
SP - 557
EP - 564
JO - BIOTECHNOLOGY ADVANCES
JF - BIOTECHNOLOGY ADVANCES
ER -