Ultra high performance liquid chromatography-electrospray ionization-tandem mass spectrometry screening method for direct analysis of designer drugs, "spice" and stimulants in oral fluid

TY - JOUR

T1 - Ultra high performance liquid chromatography-electrospray ionization-tandem mass spectrometry screening method for direct analysis of designer drugs, "spice" and stimulants in oral fluid

AU - Strano-Rossi, Sabina

AU - Anzillotti, Luca

AU - Castrignanò, Erika

AU - Romolo, Francesco Saverio

AU - Chiarotti, Marcello

PY - 2012/10/5

Y1 - 2012/10/5

N2 - An ultra high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) screening method for the direct analysis in oral fluid (OF) of 24 drugs, including new synthetic cannabinoids and so-called "smart" designer drugs, in a single chromatographic run was set up. Benzylpiperazine, methylone, 5,6-methylenedioxy-2-aminoindane (MDAI), fenproporex, 4-fluoroamphetamine (4-FA), 4-methyl-N-ethylcathinone (4-MEC), 4-methylamphetamine (4-MA), methylbenzodioxolylbutanamine (MBDB), mephedrone, methylthioamphetamine (MTA), methylenedioxypyrovalerone (MDPV), mefenorex, nabilone, furfenorex, clobenzorex, JWH-200, AM 694, JWH-250, JWH-073, JWH-018, JWH-019, JWH-122, HU 210 and CP 47497 were determined in a chromatographic run of 9. min only with no sample pre-treatment, after addition of ISs and dilution in mobile phase A. This method is designed to be applied to 250. μL of OF sample, anyway is suitable to be used on smaller volumes (till 100. μL). LODs vary from 1. ng/mL to 20. ng/mL. No interfering peaks were observed due to similar analytes, common therapeutic drugs or endogenous compounds. Matrix effect, although present especially for mephedrone, is acceptable, allowing the detection of the compounds at the LODs described. The developed method was applied on 400 real OF samples from on-site tests performed by police officers.

AB - An ultra high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) screening method for the direct analysis in oral fluid (OF) of 24 drugs, including new synthetic cannabinoids and so-called "smart" designer drugs, in a single chromatographic run was set up. Benzylpiperazine, methylone, 5,6-methylenedioxy-2-aminoindane (MDAI), fenproporex, 4-fluoroamphetamine (4-FA), 4-methyl-N-ethylcathinone (4-MEC), 4-methylamphetamine (4-MA), methylbenzodioxolylbutanamine (MBDB), mephedrone, methylthioamphetamine (MTA), methylenedioxypyrovalerone (MDPV), mefenorex, nabilone, furfenorex, clobenzorex, JWH-200, AM 694, JWH-250, JWH-073, JWH-018, JWH-019, JWH-122, HU 210 and CP 47497 were determined in a chromatographic run of 9. min only with no sample pre-treatment, after addition of ISs and dilution in mobile phase A. This method is designed to be applied to 250. μL of OF sample, anyway is suitable to be used on smaller volumes (till 100. μL). LODs vary from 1. ng/mL to 20. ng/mL. No interfering peaks were observed due to similar analytes, common therapeutic drugs or endogenous compounds. Matrix effect, although present especially for mephedrone, is acceptable, allowing the detection of the compounds at the LODs described. The developed method was applied on 400 real OF samples from on-site tests performed by police officers.

KW - DUID

KW - Forensic toxicology

KW - Oral fluid

KW - Smart drugs

KW - Synthetic cannabinoids

KW - UHPLC-MS/MS

UR - http://www.scopus.com/inward/record.url?scp=84866042001&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2012.07.098

DO - 10.1016/j.chroma.2012.07.098

M3 - Article

C2 - 22939380

AN - SCOPUS:84866042001

SN - 0021-9673

VL - 1258

SP - 37

EP - 42

JO - Journal of Chromatography A

JF - Journal of Chromatography A

ER -